Strehin Iossif, Ambrose Winnette McIntosh, Schein Oliver, Salahuddin Afrah, Elisseeff Jennifer
Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland, USA.
J Cataract Refract Surg. 2009 Mar;35(3):567-76. doi: 10.1016/j.jcrs.2008.11.035.
To describe the synthesis of a chondroitin sulfate-polyethylene glycol (CS-PEG) adhesive and characterize its physical and biological properties in vitro and in vivo.
Johns Hopkins University and a research facility, Baltimore, Maryland, USA.
Metabolic activity (WST-1 reagent) was used to evaluate the cytocompatibility of the adhesive with rabbit primary epithelial, stromal, and endothelial cells. Full-thickness corneal incisions (3.0 mm) in ex vivo porcine eyes were sealed with the adhesive, and burst pressure was evaluated to determine the effectiveness of the material in maintaining intraocular pressure (IOP). Finally, a partial-thickness incision was made in a swine cornea and then sealed using the adhesive. Two weeks postoperatively, both eyes were enucleated and examined grossly and histologically.
In vitro results showed cytocompatibility of the tissue adhesive with corneal cells and an ability to seal full-thickness corneal incisions exposed to IOPs of 200 mm Hg and higher. Histological evidence from in vivo data confirmed that the CS-PEG material is biodegradable, induces minimal inflammatory response, resists epithelial cell ingrowth, and does not induce scar formation.
The new adhesive was effective in restoring IOP and withstanding pressures greater than 200 mm Hg after being applied to a full-thickness corneal incision. The adhesive material was biocompatible with the 3 types of cells found in corneal tissue. When the adhesive was implanted in a live swine model, no adverse side effects were observed.
描述硫酸软骨素-聚乙二醇（CS-PEG）粘合剂的合成，并在体外和体内对其物理和生物学特性进行表征。
美国马里兰州巴尔的摩市约翰·霍普金斯大学及一个研究机构。
采用代谢活性（WST-1试剂）评估该粘合剂与兔原代上皮细胞、基质细胞和内皮细胞的细胞相容性。用该粘合剂封闭离体猪眼的全层角膜切口（3.0毫米），并评估破裂压力以确定该材料维持眼内压（IOP）的有效性。最后，在猪角膜上制作部分厚度切口，然后用该粘合剂封闭。术后两周，摘除双眼并进行大体和组织学检查。
体外结果显示该组织粘合剂与角膜细胞具有细胞相容性，并且能够封闭暴露于200毫米汞柱及更高眼内压的全层角膜切口。体内数据的组织学证据证实，CS-PEG材料可生物降解，引起的炎症反应最小，能抵抗上皮细胞向内生长，且不会诱导瘢痕形成。
这种新型粘合剂在应用于全层角膜切口后，能有效恢复眼内压并承受大于200毫米汞柱的压力。该粘合剂材料与角膜组织中发现的3种细胞具有生物相容性。当该粘合剂植入活猪模型中时，未观察到不良副作用。
