Matsuda Yoshikazu, Ohsaka Kazumasa, Yamamoto Hidekazu, Jiyouraku Kouzou, Natsume Katsuhiko, Hirabayashi Shirokazu, Kounoike Masayoshi, Inoue Masaaki
Department of Integrative Physiology & Bio-System Control, Shinshu University School of Medicine, Matsumoto, Nagano, Japan.
Exp Anim. 2007 Apr;56(2):131-7. doi: 10.1538/expanim.56.131.
NARCOBIT is the first anesthetic system for mice and rats to incorporate a ventilator. Therefore, it is expected to improve the reliability of mice and rat experiments by accurately controlling and maintaining the depth of anesthesia. In this study, we used NARCOBIT for inducing inhalational anesthesia in mice and evaluated the changes in their hemodynamic parameters. ICR mice were anesthetized with 5% isoflurane and room air, followed by endotracheal intubation. Subsequently, they were mechanically ventilated, and anesthesia was maintained by 2% isoflurane for a 60-min period (maintenance state) using NARCOBIT. In study 1, the heart rate (HR) and mean arterial blood pressure (MAP) were measured. The skin blood flow (SBF) from the hind legs was continuously measured during the maintenance state. Subsequently, the concentration-dependent effects of isoflurane on MAP were examined. In study 2, blood samples were obtained from the abdominal aorta for blood gas analysis. The HR and MAP decreased after anesthesia but were stable during the maintenance state. Decreased MAP and concentration-dependent effects of isoflurane were observed. The SBF increased slightly during the maintenance state but this increase was insignificant. The blood gas analysis showed neither hypoxia nor hypercapnia. Since the use of NARCOBIT enables the anesthetic concentration of isoflurane to be easily changed, a suitable anesthesia depth can be obtained for experimental purposes. Therefore, we conclude that NARCOBIT can be used for providing inhalational anesthesia to mice.
NARCOBIT是首款用于小鼠和大鼠的配备呼吸机的麻醉系统。因此，预计它能通过精确控制和维持麻醉深度来提高小鼠和大鼠实验的可靠性。在本研究中，我们使用NARCOBIT对小鼠进行吸入麻醉，并评估其血流动力学参数的变化。将ICR小鼠用5%异氟醚和室内空气麻醉，随后进行气管插管。接着，使用NARCOBIT对其进行机械通气，并通过2%异氟醚维持麻醉60分钟（维持状态）。在研究1中，测量心率（HR）和平均动脉血压（MAP）。在维持状态下持续测量后腿的皮肤血流量（SBF）。随后，检测异氟醚对MAP的浓度依赖性效应。在研究2中，从腹主动脉采集血样进行血气分析。麻醉后HR和MAP下降，但在维持状态下保持稳定。观察到MAP下降以及异氟醚的浓度依赖性效应。维持状态下SBF略有增加，但这种增加不显著。血气分析显示既无低氧也无高碳酸血症。由于使用NARCOBIT能够轻松改变异氟醚的麻醉浓度，因此可为实验目的获得合适的麻醉深度。所以，我们得出结论，NARCOBIT可用于为小鼠提供吸入麻醉。
