Andrology Laboratory, West China Hospital, Sichuan University, Chengdu, 610000, China.
Department of Urology, Institute of Urology, West China Hospital, Sichuan University, Chengdu, 610041, China.
Sci Rep. 2017 Nov 2;7(1):14949. doi: 10.1038/s41598-017-15020-5.
Intracavernosal pressure (ICP) is gold standard for the detection of erectile function in animals, but no consensus has yet been achieved on what kind of anesthetic protocol should be applied. A total of 16 adult male Sprague-Dawley rats were randomized into two groups. In group A, chloral hydrate was injected intraperitoneally. Rats in group B were induced in 5% isoflurane for 3 min and then maintained in 1.0-1.5% isoflurane. Mean arterial pressure (MAP), respiratory rate (RR) and heart rate were monitored during all experiments. After ICP detection, tail vein and carotid artery blood were collected. The maximum ICP value, MAP and ICP/MAP ratio in group B was significantly higher than in that of group A. The RR in group A was lower than in that of group B, but the heart rate in group A was higher than in group B. There were no significant differences in both pO and pCO between groups. While the data showed that animals in group A were relatively hypoxemic. Isoflurane inhalation anesthesia in detection of erectile function could offer a relatively more stable physical state than in that under the effect of chloral hydrate intraperitoneal anesthesia. Isoflurane inhalation anesthesia is more suitable for ICP test.
海绵体内部压力（ICP）是检测动物勃起功能的金标准，但对于应应用哪种麻醉方案尚未达成共识。总共 16 只成年雄性 Sprague-Dawley 大鼠被随机分为两组。在 A 组中，腹腔内注射水合氯醛。B 组大鼠用 5%异氟烷诱导 3 分钟，然后用 1.0-1.5%异氟烷维持。在所有实验过程中监测平均动脉压（MAP）、呼吸频率（RR）和心率。ICP 检测后，采集尾静脉和颈动脉血。B 组的最大 ICP 值、MAP 和 ICP/MAP 比值明显高于 A 组。A 组的 RR 低于 B 组，但 A 组的心率高于 B 组。两组之间的 pO 和 pCO 均无显著差异。虽然数据显示 A 组的动物相对缺氧。与腹腔内注射水合氯醛的效果相比，异氟烷吸入麻醉在检测勃起功能时可提供更稳定的生理状态。异氟烷吸入麻醉更适合 ICP 测试。
