NOV（CCN3）作为人类造血干细胞或祖细胞的调节剂发挥作用。

NOV (CCN3) functions as a regulator of human hematopoietic stem or progenitor cells.

作者信息

Gupta Rajeev, Hong Dengli, Iborra Francisco, Sarno Samantha, Enver Tariq

机构信息

Medical Research Council Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, OX3 9DS, UK.

出版信息

Science. 2007 Apr 27;316(5824):590-3. doi: 10.1126/science.1136031.

DOI:10.1126/science.1136031

PMID:17463287

Abstract

Clinically successful hematopoietic cell transplantation is dependent on hematopoietic stem and progenitor cells. Here we identify the matricellular protein Nephroblastoma Overexpressed (Nov, CCN3) as being essential for their functional integrity. Nov expression is restricted to the primitive (CD34) compartments of umbilical vein cord blood, and its knockdown in these cells by lentivirus-mediated RNA interference abrogates their function in vitro and in vivo. Conversely, forced expression of Nov and addition of recombinant Nov protein both enhance primitive stem and/or progenitor activity. Taken together, our results identify Nov (CCN3) as a regulator of human hematopoietic stem or progenitor cells.

摘要

临床上成功的造血细胞移植依赖于造血干细胞和祖细胞。在此，我们确定基质细胞蛋白肾母细胞瘤过度表达蛋白（Nov，CCN3）对其功能完整性至关重要。Nov的表达仅限于脐静脉血的原始（CD34）区室，通过慢病毒介导的RNA干扰在这些细胞中敲低Nov可消除其在体外和体内的功能。相反，Nov的强制表达和重组Nov蛋白的添加均增强原始干细胞和/或祖细胞活性。综上所述，我们的结果确定Nov（CCN3）为人类造血干细胞或祖细胞的调节因子。