Radboud University Nijmegen Medical Centre, Department of Urology, Nijmegen, Netherlands.
Institute of Biomedicine, University of Helsinki, Department of Biochemistry and Developmental Biology, Helsinki, Finland.
Biomed Res Int. 2018 Jun 10;2018:9634902. doi: 10.1155/2018/9634902. eCollection 2018.
To evaluate the antitumor effect of cyclodextrin-curcumin complex (CDC) on human and rat urothelial carcinoma cells and to evaluate the effect of intravesical instillations of CDC, BCG, and the combination in the AY-F344 orthotopic bladder cancer rat model. Curcumin has anticarcinogenic activity on urothelial carcinoma and is therefore under investigation for the treatment of non-muscle invasive bladder cancer. Curcumin and BCG share immunomodulating pathways against urothelial carcinoma.
Curcumin was complexed with cyclodextrin to improve solubility. Four human urothelial carcinoma cell lines and the AY-27 rat cell line were exposed to various concentrations of CDC . For the experiment, the AY-27 orthotopic bladder cancer F344 rat model was used. Rats were treated with consecutive intravesical instillations of CDC, BCG, the combination of CDC+BCG, or NaCl as control.
CDC showed a dose-dependent antiproliferative effect on all human urothelial carcinoma cell lines tested and the rat AY-27 urothelial carcinoma cell line. Moreover, intravesical treatment with CDC and CDC+BCG results in a lower percentage of tumors (60% and 68%, respectively) compared to BCG (75%) or control (85%). This difference with placebo was not statistically significant (p=0.078 and 0.199, respectively). However, tumors present in the placebo and BCG-treated rats were generally of higher stage.
Cyclodextrin-curcumin complex showed an antiproliferative effect on human and rat urothelial carcinoma cell lines . In the aggressive orthotopic bladder cancer rat model, we observed a promising effect of CDC treatment and CDC in combination with BCG.
评估环糊精-姜黄素复合物（CDC）对人及大鼠尿路上皮癌细胞的抗肿瘤作用，并评估 CDC、BCG 及二者联合膀胱内灌注在 AY-F344 原位膀胱癌大鼠模型中的作用。姜黄素对尿路上皮癌具有抗癌活性，因此正在研究其用于非肌层浸润性膀胱癌的治疗。姜黄素和 BCG 具有针对尿路上皮癌的免疫调节途径。
用环糊精将姜黄素进行包合以提高其溶解度。将四种人尿路上皮癌细胞系和 AY-27 大鼠细胞系暴露于不同浓度的 CDC 下。在该实验中，使用 AY-27 原位膀胱癌 F344 大鼠模型。对大鼠进行连续的膀胱内灌注 CDC、BCG、CDC+BCG 联合治疗或 NaCl 作为对照。
CDC 对所有测试的人尿路上皮癌细胞系和大鼠 AY-27 尿路上皮癌细胞系均表现出剂量依赖性的抗增殖作用。此外，与 BCG（75%）或对照（85%）相比，膀胱内给予 CDC 和 CDC+BCG 可使肿瘤百分比降低（分别为 60%和 68%）。与安慰剂相比，这种差异无统计学意义（分别为 p=0.078 和 0.199）。然而，安慰剂和 BCG 治疗组的肿瘤通常处于更高的分期。
环糊精-姜黄素复合物对人及大鼠尿路上皮癌细胞系表现出抗增殖作用。在侵袭性原位膀胱癌大鼠模型中，我们观察到 CDC 治疗和 CDC 联合 BCG 的治疗效果有一定的前景。
