Wang Han-Ching, Wang Hao-Ching, Kou Guang-Hsiung, Lo Chu-Fang, Huang Wei-Pang
Institute of Zoology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan.
Dis Aquat Organ. 2007 Mar 13;74(3):179-89. doi: 10.3354/dao074179.
This study investigates white spot syndrome virus (WSSV) gene expression levels in the cells of 2 hosts (Penaeus monodon and Litopenaeus vannamei). Microarray and expressed sequence tag (EST) analysis of the mRNA profiles in WSSV-infected P. monodon cells were used to identify WSSV genes that were very highly expressed. Results showed that the mRNA of the WSSV icp11 gene consistently had the highest copy number of all (3x higher than the major envelope protein, VP28). At the protein level in WSSV-infected L. vannamei, 2-dimensional gel analysis and liquid chromatography-nano-electrospray ionization tandem mass spectrometry (LC-nanoESI-MS/MS) protein identification also showed that this WSSV non-structural protein has the highest expression levels reported to date. ICP11 is capable of self-multimerization, and it becomes located in both the cytoplasm and nucleus of the host cell. These data suggest that ICP11 plays an important, but presently unknown, role during viral infection, and that expression of the WSSV icp11 gene/WSSV ICP11 protein is potentially a good and diagnostically useful indicator of WSSV infection.
本研究调查了白斑综合征病毒（WSSV）在两种宿主（斑节对虾和凡纳滨对虾）细胞中的基因表达水平。利用微阵列和表达序列标签（EST）分析WSSV感染的斑节对虾细胞中的mRNA谱，以鉴定高表达的WSSV基因。结果表明，WSSV icp11基因的mRNA拷贝数始终是所有基因中最高的（比主要包膜蛋白VP28高3倍）。在WSSV感染的凡纳滨对虾的蛋白质水平上，二维凝胶分析和液相色谱-纳升电喷雾电离串联质谱（LC-nanoESI-MS/MS）蛋白质鉴定也表明，这种WSSV非结构蛋白的表达水平是迄今为止报道的最高水平。ICP11能够自我多聚化，并定位于宿主细胞的细胞质和细胞核中。这些数据表明，ICP11在病毒感染过程中发挥着重要但目前未知的作用，并且WSSV icp11基因/WSSV ICP11蛋白的表达可能是WSSV感染的一个良好且具有诊断价值的指标。
