一种基于ICP11依赖的免疫磁珠还原（IMR）分析的新型虾白斑综合征病毒检测平台。
A Novel Detection Platform for Shrimp White Spot Syndrome Virus Using an ICP11-Dependent Immunomagnetic Reduction (IMR) Assay.
作者信息
Liu Bing-Hsien, Lin Yu-Chen, Ho Chia-Shin, Yang Che-Chuan, Chang Yun-Tsui, Chang Jui-Feng, Li Chun-Yuan, Cheng Cheng-Shun, Huang Jiun-Yan, Lee Yen-Fu, Hsu Ming-Hung, Lin Feng-Chun, Wang Hao-Ching, Lo Chu-Fang, Yang Shieh-Yueh, Wang Han-Ching
机构信息
MagQu Co., Ltd., New Taipei City 231, Taiwan.
Institute of Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
出版信息
PLoS One. 2015 Sep 18;10(9):e0138207. doi: 10.1371/journal.pone.0138207. eCollection 2015.
Shrimp white spot disease (WSD), which is caused by white spot syndrome virus (WSSV), is one of the world's most serious shrimp diseases. Our objective in this study was to use an immunomagnetic reduction (IMR) assay to develop a highly sensitive, automatic WSSV detection platform targeted against ICP11 (the most highly expressed WSSV protein). After characterizing the magnetic reagents (Fe3O4 magnetic nanoparticles coated with anti ICP11), the detection limit for ICP11 protein using IMR was approximately 2 x 10(-3) ng/ml, and the linear dynamic range of the assay was 0.1~1 x 10(6) ng/ml. In assays of ICP11 protein in pleopod protein lysates from healthy and WSSV-infected shrimp, IMR signals were successfully detected from shrimp with low WSSV genome copy numbers. We concluded that this IMR assay targeting ICP11 has potential for detecting the WSSV.
由白斑综合征病毒（WSSV）引起的对虾白斑病（WSD）是世界上最严重的对虾疾病之一。本研究的目的是使用免疫磁珠法（IMR）开发一个针对ICP11（WSSV中表达量最高的蛋白）的高灵敏度、自动化WSSV检测平台。在对磁性试剂（包被抗ICP11的Fe3O4磁性纳米颗粒）进行表征后，使用IMR检测ICP11蛋白的检测限约为2×10（-3）ng/ml，该检测方法的线性动态范围为0.1~1×10（6）ng/ml。在对健康和WSSV感染对虾的腹足蛋白裂解物中的ICP11蛋白进行检测时，成功地从WSSV基因组拷贝数较低的对虾中检测到了IMR信号。我们得出结论，这种针对ICP11的IMR检测方法具有检测WSSV的潜力。
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