Ognjanović B I, Marković S D, Pavlović S Z, Žikić R V, Stajn A S, Saičić Z S
Institute of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, Kragujevac, Serbia.
Physiol Res. 2008;57(3):403-411. doi: 10.33549/physiolres.931197. Epub 2007 Apr 25.
The effects of selenium (Se) on antioxidant defense system in liver and kidneys of rats with cadmium (Cd)-induced toxicity were examined. Cd exposure (15 mg Cd/kg b.m./day as CdCl(2) for 4 weeks) resulted in increased lipid peroxidation (LP) in both organs (p<0.005 and p<0.01). Vitamin C (Vit C) was decreased in the liver (p<0.005), whereas vitamin E (Vit E) was increased in the liver and kidneys (p<0.005 and p<0.05) of Cd-exposed animals. Superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were decreased in both tissues (p<0.05 and p<0.005), whereas catalase (CAT) activity was decreased only in liver (p<0.005). Glutathione S-transferase (GST) increased in both tissues (p<0.005 and p<0.01). Treatment with Se (0.5 mg Se/kg b.m./day as Na(2)SeO(3) for 4 weeks) significantly increased liver and kidneys SOD and GSH-Px activities (p<0.05 to p<0.005), as well as CAT and GST activities only in the liver (p<0.01). In animals exposed to Se, both the concentrations of Vit C (p<0.01) and Vit E (p<0.005) were increased in both tissues. Co-treatment with Se resulted in reversal of oxidative stress with significant decline in analyzed tissues Cd burden. Our results show that Se may ameliorate Cd-induced oxidative stress by decreasing LP and altering antioxidant defense system in rat liver and kidneys and that Se demonstrates the protective effect from cadmium-induced oxidative damage.
研究了硒（Se）对镉（Cd）诱导毒性的大鼠肝脏和肾脏抗氧化防御系统的影响。镉暴露（以氯化镉形式，15毫克镉/千克体重/天，持续4周）导致两个器官的脂质过氧化（LP）增加（p<0.005和p<0.01）。镉暴露动物的肝脏中维生素C（Vit C）降低（p<0.005），而肝脏和肾脏中的维生素E（Vit E）增加（p<0.005和p<0.05）。超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性在两个组织中均降低（p<0.05和p<0.005），而过氧化氢酶（CAT）活性仅在肝脏中降低（p<0.005）。谷胱甘肽S-转移酶（GST）在两个组织中均增加（p<0.005和p<0.01）。用硒（以亚硒酸钠形式，0.5毫克硒/千克体重/天，持续4周）处理显著增加了肝脏和肾脏的SOD和GSH-Px活性（p<0.05至p<0.005），以及仅在肝脏中的CAT和GST活性（p<0.01）。在暴露于硒的动物中两个组织中维生素C（p<0.01）和维生素E（p<0.005）的浓度均增加。硒联合处理导致氧化应激逆转，分析组织中的镉负荷显著下降。我们的结果表明，硒可能通过降低脂质过氧化并改变大鼠肝脏和肾脏中的抗氧化防御系统来减轻镉诱导的氧化应激，并且硒显示出对镉诱导的氧化损伤的保护作用。
