Institute for Biological Research "Siniša Stanković"-National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia.
Group for Biomedical Engineering and Nanobiotechnology, Institute of Technical Sciences of the Serbian Academy of Sciences and Arts (SASA), Kneza Mihaila 35/IV, 11000 Belgrade, Serbia.
Int J Mol Sci. 2022 Oct 28;23(21):13068. doi: 10.3390/ijms232113068.
The chemical element selenium (Se) is a nonmetal that is in trace amounts indispensable for normal cellular functioning. During pregnancy, a low Se status can increase the risk of oxidative stress. However, elevated concentrations of Se in the body can also cause oxidative stress. This study aimed to compare the effects of BSA-stabilized Se nanoparticles (SeNPs, Se) (BSA-bovine serum albumin) and inorganic sodium selenite (NaSe, Se) supplementation on the histological structure of the placenta, oxidative stress parameters and the total placental Se concentration of Wistar rats during pregnancy. Pregnant females were randomized into four groups: (i) intact controls; (ii) controls that were dosed by daily oral gavage with 8.6% bovine serum albumin (BSA) and 0.125 M vit C; (iii) the SeNP group that was administered 0.5 mg of SeNPs stabilized with 8.6% BSA and 0.125 M vit C/kg bw/day by oral gavage dosing; (iv) the NaSe group, gavage dosed with 0.5 mg NaSeO/kg bw/day. The treatment of pregnant females started on gestational day one, lasted until day 20, and on day 21 of gestation, the fetuses with the placenta were removed from the uterus. Our findings show that the mode of action of equivalent concentrations of Se in SeNPs and NaSe depended on its redox state and chemical structure. Administration of SeNPs (Se) increased fetal lethality and induced changes in the antioxidative defense parameters in the placenta. The accumulation of Se in the placenta was highest in SeNP-treated animals. All obtained data indicate an increased bioavailability of Se in its organic nano form and Se redox state in comparison to its inorganic sodium selenite form and Se redox state.
化学元素硒（Se）是一种非金属元素，在痕量下对正常细胞功能是不可或缺的。在怀孕期间，硒状态低会增加氧化应激的风险。然而，体内硒浓度升高也会引起氧化应激。本研究旨在比较 BSA 稳定的硒纳米颗粒（SeNPs，Se）（BSA-牛血清白蛋白）和无机亚硒酸钠（NaSe，Se）补充剂对怀孕 Wistar 大鼠胎盘组织学结构、氧化应激参数和胎盘总硒浓度的影响。将怀孕雌性随机分为四组：（i）完整对照组；（ii）对照组每日口服 8.6%牛血清白蛋白（BSA）和 0.125 M 维生素 C；（iii）SeNP 组，经口灌胃给予 0.5 mg 用 8.6% BSA 和 0.125 M 维生素 C/kg bw/day 稳定的 SeNPs；（iv）NaSe 组，每日口服 0.5 mg/kg bw/day NaSeO。从妊娠第 1 天开始对怀孕雌性进行治疗，持续到第 20 天，在妊娠第 21 天，从子宫中取出带有胎盘的胎儿。我们的研究结果表明，以等效浓度存在的 Se 在 SeNPs 和 NaSe 中的作用方式取决于其氧化还原状态和化学结构。SeNPs（Se）的给药增加了胎儿死亡率，并诱导胎盘抗氧化防御参数发生变化。用 SeNP 处理的动物中胎盘内硒的积累最高。所有获得的数据表明，与无机亚硒酸钠形式和 Se 氧化还原状态相比，Se 的有机纳米形式及其 Se 氧化还原状态具有更高的生物利用度。
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