Tilley Simon, Bolland Benjamin J R F, Partridge Kris, New Andrew M R, Latham Jeremy M, Dunlop Douglas G, Oreffo Richard O C
Bone & Joint Research Group, Developmental Origins of Health and Disease, University of Southampton, Southampton, UK.
Regen Med. 2006 Sep;1(5):685-92. doi: 10.2217/17460751.1.5.685.
Human bone marrow contains bone progenitor cells that arise from multipotent mesenchymal stem cells. Seeding bone progenitor cells onto a scaffold can produce a 3D living composite with significant mechanical and biological potential. This article details laboratory and clinical findings from two clinical cases, where different proximal femoral conditions were treated using impacted allograft augmented with marrow-derived autogenous progenitor cells. Autologous bone marrow was seeded onto highly washed morselized allograft and impacted. Samples of the impacted graft were also taken for ex vivo analysis. Both patients made an uncomplicated clinical recovery. Imaging confirmed defect filling with encouraging initial graft incorporation. Histochemical and alkaline phosphatase staining demonstrated that a live composite graft with osteogenic activity had been introduced into the defects. These studies demonstrate that marrow-derived cells can adhere to highly washed morselized allograft, survive the impaction process and proliferate with an osteoblastic phenotype, thus creating a living composite.
人类骨髓中含有源自多能间充质干细胞的骨祖细胞。将骨祖细胞接种到支架上可产生具有显著机械和生物学潜力的三维活性复合材料。本文详细介绍了两个临床病例的实验室和临床研究结果，其中使用骨髓来源的自体祖细胞增强的嵌压异体骨治疗了不同的股骨近端疾病。将自体骨髓接种到高度清洗过的碎异体骨上并进行嵌压。还采集了嵌压移植物的样本进行体外分析。两名患者均顺利康复。影像学检查证实缺损得到填充，且初始移植物融合情况良好。组织化学和碱性磷酸酶染色表明，具有成骨活性的活性复合材料移植物已被植入缺损处。这些研究表明，骨髓来源的细胞可以附着在高度清洗过的碎异体骨上，在嵌压过程中存活并以成骨细胞表型增殖，从而形成活性复合材料。
