Dias Ana Karina Kerche, Aoki Sérgio Moraes, Garcia José Fernando, Nunes Cáris Maroni
Department of Animal Health and Production, São Paulo State University-UNESP, Campus Araçatuba, Rua Clóvis Pestana, 793, Jd. D. Amélia, Araçatuba, SP 16050-680, Brazil.
Exp Parasitol. 2007 Sep;117(1):9-12. doi: 10.1016/j.exppara.2007.03.005. Epub 2007 Mar 16.
Cysticercosis is one of the most important zoonosis, not only because of the effects on animal health and its economic consequences, but also due to the serious danger it poses to humans. The two main parasites involved in the taeniasis-cysticercosis complex in Brazil are Taenia saginata and Taenia solium. Differentiating between these two parasites is important both for disease control and for epidemiological studies. The purpose of this work was to identify genetic markers that could be used to differentiate these parasites. Out of 120 oligonucleotide decamers tested in random amplified polymorphic DNA (RAPD) assays, 107 were shown to discriminate between the two species of Taenia. Twenty-one DNA fragments that were specific for each species of Taenia were chosen for DNA cloning and sequencing. Seven RAPD markers were converted into sequence characterized amplified region (SCAR) markers with two specific for T. saginata and five specific for T. solium as shown by agarose gel electrophoresis. These markers were developed as potential tools to differentiate T. solium from T. saginata in epidemiological studies.
囊尾蚴病是最重要的人畜共患病之一，不仅因为它对动物健康的影响及其经济后果，还因为它对人类构成的严重危险。巴西牛带绦虫病-囊尾蚴病复合体中涉及的两种主要寄生虫是牛带绦虫和猪带绦虫。区分这两种寄生虫对于疾病控制和流行病学研究都很重要。这项工作的目的是鉴定可用于区分这些寄生虫的遗传标记。在随机扩增多态性DNA（RAPD）分析中测试的120个十聚体寡核苷酸中，有107个显示出能区分两种绦虫。选择了21个每种绦虫特有的DNA片段进行DNA克隆和测序。如琼脂糖凝胶电泳所示，7个RAPD标记被转化为序列特征性扩增区域（SCAR）标记，其中2个对牛带绦虫特异，5个对猪带绦虫特异。这些标记被开发为在流行病学研究中区分猪带绦虫和牛带绦虫的潜在工具。
