Rodriguez-Hidalgo R, Geysen D, Benítez-Ortiz W, Geerts S, Brandt J
Laboratorio de Immunodiagnostico e Investigación, Facultad de Medicina Veterinaria y Zootecnia, Universidad Central del Ecuador, Quito, Ecuador.
J Parasitol. 2002 Oct;88(5):1007-11. doi: 10.1645/0022-3395(2002)088[1007:COCTTD]2.0.CO;2.
Given the constraints of classical diagnostic methods, i.e., morphological and isoenzymatic studies of proglottids, a polymerase chain reaction test complemented with restriction enzyme analysis has been modified by redesigning one of the primers to reduce nonspecific amplifications experienced when using field samples. The use of these new, highly cestode-specific primers and the restriction enzyme Ddel led to the development of a diagnostic assay that clearly distinguishes between Taenia saginata and T. solium proglottids in field samples. This assay confirms the presence of T. saginata in Ecuador. DNA amplification of some of these taeniids showed different patterns, suggesting the possibility that strain differences exist. These results demonstrate the need for development of useful molecular assays as reliable tools for epidemiological studies on cestodes.
鉴于传统诊断方法（即对绦虫节片进行形态学和同工酶研究）的局限性，通过重新设计其中一个引物，对聚合酶链反应试验辅以限制性酶切分析进行了改进，以减少在使用野外样本时出现的非特异性扩增。使用这些新型、高度绦虫特异性的引物和限制性酶Ddel，开发出了一种诊断检测方法，可在野外样本中清晰区分牛带绦虫和猪带绦虫的节片。该检测方法证实了厄瓜多尔存在牛带绦虫。对其中一些带绦虫的DNA扩增显示出不同模式，这表明存在菌株差异的可能性。这些结果表明，需要开发有用的分子检测方法，作为绦虫流行病学研究的可靠工具。
