Sangrador-Vegas A, Stanton C, van Sinderen D, Fitzgerald G F, Ross R P
Moorepark Food Research Centre, Teagasc, Fermoy, County Cork, Ireland.
Plasmid. 2007 Sep;58(2):140-7. doi: 10.1016/j.plasmid.2007.02.004. Epub 2007 Apr 30.
Bifidobacterium pseudolongum subsp. globosum DPC479 is an intestinally-derived strain which contains a plasmid, pASV479, 4.8 kb in size. This plasmid has a G + C content of 59% and contains six open reading frames (ORFs), four of which are cryptic. The other two ORFs have 47% and 54% identity, respectively, to the replication and FtsK-like proteins found in a Bifidobacterium breve NCFB 2258 plasmid, indicating that these plasmids, though isolated from differing Bifidobacterium species, are related. Using this plasmid as a backbone, an expression vector, pBIFRIBO, was constructed which exploits a bifidobacteria rRNA promoter.
伪长双歧杆菌球亚种DPC479是一种源自肠道的菌株，它含有一个大小为4.8 kb的质粒pASV479。该质粒的G + C含量为59%，包含六个开放阅读框（ORF），其中四个是隐蔽的。另外两个ORF分别与短双歧杆菌NCFB 2258质粒中的复制蛋白和FtsK样蛋白具有47%和54%的同一性，这表明这些质粒虽然是从不同的双歧杆菌物种中分离出来的，但它们是相关的。以该质粒为骨架，构建了一个利用双歧杆菌rRNA启动子的表达载体pBIFRIBO。
