Tan Thean Yen, Ng Lily Siew Yong, Poh Karen
Laboratory Medicine Services, Changi General Hospital, Singapore 529889, Singapore.
Diagn Microbiol Infect Dis. 2007 Jul;58(3):357-61. doi: 10.1016/j.diagmicrobio.2007.02.008. Epub 2007 Apr 30.
Treatment options are increasingly limited for carbapenem-resistant Acinetobacter baumannii. This study set out to determine the in vitro susceptibility of multiresistant Acinetobacter spp. to colistin, minocycline, and rifampicin using agar dilution, and to compare the accuracy of testing results obtained from an automated susceptibility testing system (Vitek 2). MICs for colistin, minocycline, and rifampicin were obtained by agar dilution and Vitek 2 for 44 unrelated strains of multiresistant Acinetobacter spp. All tested strains were susceptible to colistin, whereas 61% were susceptible to minocycline and 52% were susceptible to rifampicin. Results for colistin testing showed categoric agreement between Vitek 2 and agar dilution, with no false-resistance reported by Vitek 2. However, there was a poor agreement for minocycline and rifampicin when results obtained from Vitek 2 testing were compared with those obtained by agar dilution.
对于耐碳青霉烯类鲍曼不动杆菌，治疗选择越来越有限。本研究旨在使用琼脂稀释法测定多重耐药不动杆菌属对黏菌素、米诺环素和利福平的体外敏感性，并比较自动药敏试验系统（Vitek 2）获得的检测结果的准确性。通过琼脂稀释法和Vitek 2对44株不相关的多重耐药不动杆菌属菌株测定了黏菌素、米诺环素和利福平的最低抑菌浓度（MIC）。所有测试菌株对黏菌素敏感，而61%对米诺环素敏感，52%对利福平敏感。黏菌素检测结果显示Vitek 2和琼脂稀释法之间完全一致，Vitek 2未报告假耐药情况。然而，将Vitek 2检测结果与琼脂稀释法获得的结果进行比较时，米诺环素和利福平的一致性较差。
