Kohnoe S, Emi Y, Takahashi I, Yoshida M, Maehara Y, Sugimachi K
Cancer Center, Kyushu University Hospital, Fukuoka, Japan.
Anticancer Res. 1991 Jul-Aug;11(4):1401-4.
To clarify the influence of hypoxic and acidic environments on the cytotoxicity of mitomycin C (MMC) and carboquone (CQ), the cytotoxicity was assessed based on decreases in intracellular ATP levels of HeLa cells exposed to the drugs at various acidic pH (pH 5.8-6.8) and low oxygen tension (5% and 0% O2) in vitro. Hypoxia and acidity individually increased the cytotoxicity of MMC, although hypoxia no longer increased it under acidic conditions. On the other hand, the cytotoxicity of CQ was enhanced in the presence of hypoxic and acidic conditions, and even more so with their combination. We conclude that acidity is a determining factor and excels hypoxia in influencing the cytotoxicity of MMC and that these two factors act synergistically on CQ.
为阐明缺氧和酸性环境对丝裂霉素C（MMC）和卡波醌（CQ）细胞毒性的影响，在体外不同酸性pH值（pH 5.8 - 6.8）和低氧张力（5%和0% O₂）条件下，基于暴露于这些药物的HeLa细胞内ATP水平的降低来评估细胞毒性。缺氧和酸性各自增加了MMC的细胞毒性，尽管在酸性条件下缺氧不再增加其细胞毒性。另一方面，CQ的细胞毒性在缺氧和酸性条件下增强，且二者联合时增强更明显。我们得出结论，酸性是影响MMC细胞毒性的决定性因素且比缺氧更显著，并且这两个因素对CQ起协同作用。
