Herman Rod A, Woolhiser Michael M, Ladics Gregory S, Korjagin Valerie A, Schafer Barry W, Storer Nicholas P, Green Susan B, Kan Lynn
Dow AgroSciences LLC, 9330 Zionsville Road, Indianapolis, IN 46268, USA.
Int J Food Sci Nutr. 2007 Mar;58(2):125-41. doi: 10.1080/09637480601149640.
Stability in simulated gastric fluid has been suggested as a parameter for consideration in the allergenicity assessment of transgenic proteins. However, the relationship between the stability of proteins in simulated gastric fluid and allergenicity has been inconsistent among studies conducted with reference allergens and non-allergens. Differences in laboratory methods and data interpretation have been implicated as possible causes for conflicting study results. We attempted to mitigate some of the methodological inconsistencies among laboratory methods by applying a kinetic interpretation to results of digestion experiments conducted with a set of known allergens and putative non-allergens. We found that pepsinolysis in simulated gastric fluid generally followed an exponential (pseudo-first-order) pattern of decay, at least during the terminal (slower) phase of digestion, allowing the calculation of digestion half-lives. While digestibility estimates were reproducible and robust, results for the proteins evaluated in this study did not support a significant association between stability in simulated gastric fluid and allergenicity.
模拟胃液中的稳定性已被建议作为转基因蛋白致敏性评估中需考虑的一个参数。然而，在针对参考过敏原和非过敏原进行的研究中，蛋白质在模拟胃液中的稳定性与致敏性之间的关系并不一致。实验室方法和数据解读的差异被认为是导致研究结果相互矛盾的可能原因。我们试图通过对一组已知过敏原和假定非过敏原进行消化实验的结果应用动力学解释，来减少实验室方法之间的一些方法学不一致性。我们发现，模拟胃液中的胃蛋白酶消化通常遵循指数（伪一级）衰减模式，至少在消化的末期（较慢）阶段是这样，这使得能够计算消化半衰期。虽然消化率估计值具有可重复性和稳健性，但本研究中评估的蛋白质结果并不支持模拟胃液中的稳定性与致敏性之间存在显著关联。
