Giraldo Paulo C, Babula Oksana, Gonçalves Ana Katherine S, Linhares Iara M, Amaral Rose Luce, Ledger William J, Witkin Steven S
Department of Gynecology and Obstetrics, The State University of Campinas, School of Medical Sciences, São Paulo, Brazil.
Obstet Gynecol. 2007 May;109(5):1123-8. doi: 10.1097/01.AOG.0000260386.17555.a5.
To evaluate associations between polymorphisms in the gene coding for mannose-binding lectin (MBL) and the diagnosis of acute or recurrent vulvovaginal candidiasis and bacterial vaginosis
Women at two outpatient clinics in Brazil filled out a questionnaire and were examined for the presence of vulvovaginal candidiasis or bacterial vaginosis. A buccal swab was blindly tested for codons 54 and 57 MBL2 gene polymorphisms by polymerase chain reaction and endonuclease digestion.
A total of 177 women were enrolled. Vulvovaginal candidiasis was identified in 78 (44.1%) women, 33 (18.6%) had bacterial vaginosis, and 66 (37.3%) were normal controls. Recurrent vulvovaginal candidiasis was present in 50 (64.1%) of the women with vulvovaginal candidiasis; 20 (60.6%) of the bacterial vaginosis patients had recurrent disease. Vulvovaginal candidiasis was associated with white race (P=.007), bacterial vaginosis was associated with nonwhite race (P=.05), and both were associated with a history of allergy (P< or =.02) and having sexual intercourse at least three times a week (P<.001). Carriage of the variant MBL2 codon 54 allele B was more frequent in women with recurrent vulvovaginal candidiasis (25.0%) than in the women with acute vulvovaginal candidiasis (17.9%) or controls (10.6%) (P=.004). Allele B was also more prevalent in women with recurrent bacterial vaginosis (22.5%) than in those with acute bacterial vaginosis (0%) (P=.009). The MBL2 codon 57 polymorphism was infrequent and not associated with vulvovaginal candidiasis or bacterial vaginosis.
The incidence of vulvovaginal candidiasis and bacterial vaginosis differs by ethnicity in Brazilian women. The MBL2 codon 54 gene polymorphism is associated with both recurrent vulvovaginal candidiasis and recurrent bacterial vaginosis.
评估甘露糖结合凝集素（MBL）编码基因多态性与急性或复发性外阴阴道念珠菌病及细菌性阴道病诊断之间的关联。
巴西两家门诊诊所的女性填写问卷，并接受外阴阴道念珠菌病或细菌性阴道病检查。通过聚合酶链反应和核酸内切酶消化对颊拭子进行盲法检测，以确定MBL2基因第54和57密码子的多态性。
共纳入177名女性。78名（44.1%）女性被诊断为外阴阴道念珠菌病，33名（18.6%）患有细菌性阴道病，66名（37.3%）为正常对照。50名（64.1%）外阴阴道念珠菌病女性患有复发性外阴阴道念珠菌病；20名（60.6%）细菌性阴道病患者患有复发性疾病。外阴阴道念珠菌病与白种人相关（P = 0.007），细菌性阴道病与非白种人相关（P = 0.05），两者均与过敏史（P≤0.02）和每周至少性交三次相关（P < 0.001）。复发性外阴阴道念珠菌病女性中变异型MBL2第54密码子等位基因B的携带频率（25.0%）高于急性外阴阴道念珠菌病女性（17.9%）或对照组（10.6%）（P = 0.004）。等位基因B在复发性细菌性阴道病女性中（22.5%）也比急性细菌性阴道病女性中（0%）更普遍（P = 0.009）。MBL2第57密码子多态性少见，且与外阴阴道念珠菌病或细菌性阴道病无关。
巴西女性中外阴阴道念珠菌病和细菌性阴道病的发病率因种族而异。MBL2第54密码子基因多态性与复发性外阴阴道念珠菌病和复发性细菌性阴道病均相关。
