Sampath Rangarajan, Hall Thomas A, Massire Christian, Li Feng, Blyn Lawrence B, Eshoo Mark W, Hofstadler Steven A, Ecker David J
Ibis Biosciences Inc, Subsidiary of Isis Pharmaceuticals, Carlsbad, CA 92008, USA.
Ann N Y Acad Sci. 2007 Apr;1102(1):109-20. doi: 10.1196/annals.1408.008.
Newly emergent infectious diseases are a global public health problem. The population dense regions of Southeast Asia are the epicenter of many emerging diseases, as evidenced by the outbreak of Nipah, SARS, avian influenza (H5N1), Dengue, and enterovirus 71 in this region in the past decade. Rapid identification, epidemiologic surveillance, and mitigation of transmission are major challenges in ensuring public health safety. Here we describe a powerful new approach for infectious disease surveillance that is based on polymerase chain reaction (PCR) to amplify nucleic acid targets from large groupings of organisms, electrospray ionization mass spectrometry (ESI-MS) for accurate mass measurements of the PCR products, and base composition signature analysis to identify organisms in a sample. This approach is capable of automated analysis of more than 1,500 PCR reactions a day. It is applicable to the surveillance of bacterial, viral, fungal, or protozoal pathogens and will facilitate rapid characterization of known and emerging pathogens.
新出现的传染病是一个全球公共卫生问题。东南亚人口密集地区是许多新出现疾病的中心，过去十年该地区爆发的尼帕病毒、严重急性呼吸综合征、禽流感（H5N1）、登革热和肠道病毒71型就是明证。快速识别、流行病学监测以及传播的缓解是确保公共卫生安全的主要挑战。在此，我们描述了一种强大的传染病监测新方法，该方法基于聚合酶链反应（PCR）从大量生物体中扩增核酸靶标，利用电喷雾电离质谱（ESI-MS）对PCR产物进行精确质量测量，并通过碱基组成特征分析来识别样本中的生物体。这种方法能够每天自动分析超过1500个PCR反应。它适用于细菌、病毒、真菌或原生动物病原体的监测，并将有助于对已知和新出现的病原体进行快速鉴定。
