荧光标记且内部淬灭的UDP-半乳糖探针的合成。
Synthesis of fluorescently labelled and internally quenched UDP-Gal probes.
作者信息
Schweizer Frank
机构信息
Department of Chemistry, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2.
出版信息
Carbohydr Res. 2007 Sep 3;342(12-13):1831-40. doi: 10.1016/j.carres.2007.03.029. Epub 2007 Apr 11.
The preparation of fluorescently labelled and internally quenched UDP-Gal probes bearing a fluorescence emitter and a quencher is described. The rate of transfer using several galactosyltransferases was examined. Our results demonstrate that galactose-modified, sugar-nucleotide-modified and double modified UDP-Gal analogues are recognized as weak substrates by blood group B alpha-(1-->3) galactosyltransferase, alpha-(1-->3) galactosyltransferase and milk bovine beta-(1-->4) galactosyltransferase.
本文描述了带有荧光发射体和猝灭剂的荧光标记且内部猝灭的UDP-半乳糖探针的制备。研究了使用几种半乳糖基转移酶时的转移速率。我们的结果表明，半乳糖修饰、糖核苷酸修饰和双重修饰的UDP-半乳糖类似物被B型血α-(1→3)半乳糖基转移酶、α-(1→3)半乳糖基转移酶和牛奶牛β-(1→4)半乳糖基转移酶识别为弱底物。
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