Castelucci Simone, de Paula Rogerio Alexandre, Ambrosio Sérgio Ricardo, Arakawa Nilton Syogo, de Lira Simone Possedente, Faccioli Lúcia Helena, Da Costa Fernando Batista
Laboratório de Farmacognosia, Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP), Universidade de São Paulo (USP), Av. do Café s/n, 14040-903 Ribeirão Preto, SP, Brazil.
J Ethnopharmacol. 2007 May 30;112(1):192-8. doi: 10.1016/j.jep.2007.01.037. Epub 2007 Feb 24.
The tea prepared from leaves and thorns of Dasyphyllum brasiliensis (Asteraceae) is used in the traditional medicine in Brazil for the treatment of oral and oropharyngeal diseases. In this study, we investigated the anti-inflammatory activity of this plant. The aqueous crude extract (ACE), the methanol-water (MeOH-H(2)O) fraction obtained by solvent partition and its fractionation products were evaluated for their anti-inflammatory activities on acute peritonitis induced by beta-glucan from the cell walls of Histoplasma capsulatum. The antiedematogenic activity was also tested using the carrageenan-induced paw edema assay in mice. Oral administration of 100 and 300mg/kg of the ACE in mice caused a significant reduction of neutrophil and eosinophil recruitment in the acute peritonitis assay. In addition, ACE at 300mg/kg inhibited the number of mononuclear cells recruitment. The MeOH-H(2)O fraction and its fractionation products (all at 100mg/kg) also presented anti-inflammatory activities, confirmed by the inhibition of cells recruited to the peritoneal cavity. ACE at 100mg/kg did not show any significant reduction of the edema in the mice paw injected with carrageenan. These data together suggest that Dasyphyllum brasiliensis presents significant anti-inflammatory activity, thus supporting the popular use of the tea in the treatment of inflammatory diseases.
用巴西刺冠菊（菊科）的叶子和刺制成的茶在巴西传统医学中用于治疗口腔和口咽疾病。在本研究中，我们调查了这种植物的抗炎活性。评估了水粗提物（ACE）、通过溶剂分配获得的甲醇 - 水（MeOH - H₂O）馏分及其分级产物对荚膜组织胞浆菌细胞壁β - 葡聚糖诱导的急性腹膜炎的抗炎活性。还使用角叉菜胶诱导的小鼠足肿胀试验测试了抗水肿活性。在小鼠中口服100和300mg/kg的ACE导致急性腹膜炎试验中中性粒细胞和嗜酸性粒细胞募集显著减少。此外，300mg/kg的ACE抑制了单核细胞募集数量。MeOH - H₂O馏分及其分级产物（均为100mg/kg）也表现出抗炎活性，这通过对募集到腹腔的细胞的抑制得到证实。100mg/kg的ACE对注射角叉菜胶的小鼠足肿胀没有显示出任何显著的减轻。这些数据共同表明巴西刺冠菊具有显著的抗炎活性，从而支持了这种茶在治疗炎症性疾病方面的广泛应用。
