Jones-Engel Lisa, Steinkraus Katherine A, Murray Shannon M, Engel Gregory A, Grant Richard, Aggimarangsee Nantiya, Lee Benjamin P Y-H, May Cynthia, Schillaci Michael A, Somgird Chaleamchat, Sutthipat Tulyawat, Vojtech Lucia, Zhao JianYuan, Linial Maxine L
Washington National Primate Research Center, University of Washington, Seattle, Washington, USA.
J Virol. 2007 Jul;81(14):7330-7. doi: 10.1128/JVI.00343-07. Epub 2007 May 2.
Foamy viruses (FV) are retroviruses that naturally infect many hosts, including most nonhuman primates (NHPs). Zoonotic infection by primate FV has been documented in people in Asia who reported contact with free-ranging macaques. FV transmission in Asia is a concern, given abundant human-NHP contact, particularly at monkey temples and in urban settings. We have developed three assays capable of detecting the presence of FV in Asian NHP species that are commensal with humans: enzyme-linked immunosorbent assay (ELISA), Western blot assays using recombinant viral Gag protein, and an indicator cell line that can detect macaque FV. The recombinant ELISA correlates very well with the presence of FV sequences detected by PCR. We have used these assays to demonstrate both that FV is highly prevalent among free-ranging NHPs and that seroconversion occurs at a young age in these animals. These assays should also prove useful for large-scale analysis of the prevalence of FV infections in human populations in Asia that are commensal with free-ranging NHPs.
泡沫病毒（FV）是逆转录病毒，可自然感染包括大多数非人灵长类动物（NHP）在内的许多宿主。亚洲曾有报告称接触过自由放养猕猴的人发生了灵长类动物泡沫病毒的人畜共患感染。鉴于人与非人灵长类动物有大量接触，尤其是在猴庙和城市环境中，亚洲的泡沫病毒传播令人担忧。我们开发了三种能够检测与人类共生的亚洲非人灵长类动物物种中泡沫病毒存在情况的检测方法：酶联免疫吸附测定（ELISA）、使用重组病毒Gag蛋白的蛋白质印迹分析以及一种可检测猕猴泡沫病毒的指示细胞系。重组ELISA与通过PCR检测到的FV序列的存在情况相关性非常好。我们利用这些检测方法证明，泡沫病毒在自由放养的非人灵长类动物中高度流行，并且这些动物在幼年时就会发生血清转化。这些检测方法也应被证明对大规模分析与自由放养的非人灵长类动物共生的亚洲人群中泡沫病毒感染的流行情况有用。
