Neske Florian, Blessing Kerstin, Tollmann Franz, Schubert Jörg, Rethwilm Axel, Kreth Hans Wolfgang, Weissbrich Benedikt
Institute of Virology and Immunobiology, University of Würzburg, Versbacher Str. 7, D-97078 Würzburg, Germany.
J Clin Microbiol. 2007 Jul;45(7):2116-22. doi: 10.1128/JCM.00027-07. Epub 2007 May 2.
The human bocavirus (hBoV) was first described in 2005 in respiratory tract samples. The clinical relevance of hBoV is still unclear. The aim of our study was to establish a real-time PCR assay for the detection and quantification of hBoV DNA, to apply the real-time assay for the analysis of stool and serum samples for the presence of hBoV DNA, and to perform a phylogenetic analysis of the hBoV positive samples. A total of 834 nasopharyngeal aspirates (NPA), 10 serum samples, and 31 stool samples of children with acute respiratory diseases were retrospectively tested. For phylogenetic analysis, 968 bp of the VP2 gene were sequenced from 69 hBoV-positive NPA samples. The qualitative results of the real-time hBoV PCR were in good agreement with a conventional hBoV PCR. We found that 12% of the NPA were positive for hBoV DNA. The median viral load in the NPA was 4.9 x 10(3) copies/ml (range, 2.7 x 10 degrees to 1.5 x 10(11) copies/ml). There was no difference of the hBoV load in NPA between children with or without known coinfection, but the load was significantly higher in children with bronchitis than in children with the diagnosis of febrile seizures. hBoV DNA was found in 1 of 10 serum samples and in 14 of 31 stool samples. hBoV sequence identity was >99% in the VP2 region. In conclusion, hBoV DNA can be found in NPA samples at very high titers. In addition to being found in the respiratory tract, hBoV was found in stool samples. The clinical relevance of these findings remains to be determined.
人博卡病毒（hBoV）于2005年首次在呼吸道样本中被描述。hBoV的临床相关性仍不明确。我们研究的目的是建立一种用于检测和定量hBoV DNA的实时PCR检测方法，应用该实时检测方法分析粪便和血清样本中hBoV DNA的存在情况，并对hBoV阳性样本进行系统发育分析。对834份急性呼吸道疾病儿童的鼻咽抽吸物（NPA）、10份血清样本和31份粪便样本进行了回顾性检测。为进行系统发育分析，从69份hBoV阳性NPA样本中对VP2基因的968 bp进行了测序。实时hBoV PCR的定性结果与传统hBoV PCR结果高度一致。我们发现12%的NPA样本hBoV DNA呈阳性。NPA样本中的病毒载量中位数为4.9×10³拷贝/ml（范围为2.7×10⁰至1.5×10¹¹拷贝/ml）。有无已知合并感染的儿童NPA中hBoV载量无差异，但支气管炎患儿的载量显著高于热性惊厥诊断患儿。在10份血清样本中的1份以及31份粪便样本中的14份中发现了hBoV DNA。hBoV在VP2区域的序列同一性>99%。总之，在NPA样本中可发现高滴度的hBoV DNA。除了在呼吸道中被发现外，hBoV也在粪便样本中被发现。这些发现的临床相关性仍有待确定。
