通过铜（I）催化的炔烃-叠氮化物[2 + 3]环加成反应对磁性纳米颗粒进行表面改性。
Surface modification of magnetic nanoparticle via Cu(I)-catalyzed alkyne-azide [2 + 3] cycloaddition.
作者信息
Lin Po-Chiao, Ueng Shau-Hua, Yu Sheng-Chieh, Jan Mi-Dan, Adak Avijit Kumar, Yu Ching-Ching, Lin Chun-Cheng
机构信息
Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan.
出版信息
Org Lett. 2007 May 24;9(11):2131-4. doi: 10.1021/ol070588f. Epub 2007 May 4.
The Cu(I)-catalyzed alkyne-azide [2 + 3] cycloaddition has been demonstrated to be an effective and orthogonal conjugation reaction to covalently immobilize biomolecules on magnetic nanoparticles (MNPs). The azido group on the MNP surface provides better conjugation efficiency with alkynated molecules. Moreover, the C-terminal alkynated protein was site-specifically immobilized on MNP. The protein binding activity presented by site-specific immobilization is higher than that by random amide bond formation.
铜（I）催化的炔烃-叠氮化物[2 + 3]环加成反应已被证明是一种有效且正交的共轭反应，可将生物分子共价固定在磁性纳米颗粒（MNP）上。MNP表面的叠氮基团与炔基化分子具有更好的共轭效率。此外，C端炔基化蛋白被位点特异性地固定在MNP上。通过位点特异性固定呈现的蛋白质结合活性高于通过随机酰胺键形成的活性。
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