Briñas Raymond P, Hu Minghui, Qian Luping, Lymar Elena S, Hainfeld James F
Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA.
J Am Chem Soc. 2008 Jan 23;130(3):975-82. doi: 10.1021/ja076333e. Epub 2007 Dec 23.
We developed a method in preparing size-controllable gold nanoparticles (Au NPs, 2-6 nm) capped with glutathione by varying the pH (between 5.5 and 8.0) of the solution before reduction. This method is based on the formation of polymeric nanoparticle precursors, Au(I)-glutathione polymers, which change size and density depending on the pH. Dynamic light scattering, size exclusion chromatography, and UV-vis spectroscopy results suggest that lower pH values favor larger and denser polymeric precursors and higher pH values favor smaller and less dense precursors. Consequently, the larger precursors led to the formation of larger Au NPs, whereas smaller precursors led to the formation of smaller Au NPs. Using this strategy, Au NPs functionalized with nickel(II) nitriloacetate (Ni-NTA) group were prepared by a mixed-ligand approach. These Ni-NTA functionalized Au NPs exhibited specific binding to 6x-histidine-tagged Adenovirus serotype 12 knob proteins, demonstrating their utility in biomolecular labeling applications.
我们开发了一种制备谷胱甘肽包覆的尺寸可控金纳米颗粒（Au NPs，2 - 6纳米）的方法，即在还原前改变溶液的pH值（5.5至8.0之间）。该方法基于聚合物纳米颗粒前体Au(I)-谷胱甘肽聚合物的形成，其尺寸和密度会根据pH值而变化。动态光散射、尺寸排阻色谱和紫外可见光谱结果表明，较低的pH值有利于形成更大、更致密的聚合物前体，而较高的pH值有利于形成更小、密度更低的前体。因此，较大的前体导致形成较大的Au NPs，而较小的前体导致形成较小的Au NPs。使用这种策略，通过混合配体方法制备了用镍（II）次氮基三乙酸（Ni-NTA）基团功能化的Au NPs。这些Ni-NTA功能化的Au NPs对6x-组氨酸标签的腺病毒血清型12纤突蛋白表现出特异性结合，证明了它们在生物分子标记应用中的实用性。
