Akihisa Toshihiro, Hayakawa Yosuke, Tokuda Harukuni, Banno Norihiro, Shimizu Naoto, Suzuki Takashi, Kimura Yumiko
College of Science and Technology, Nihon University, 1-8 Kanda Surugadai, Chiyoda-ku, Tokyo 101-8308, Japan.
J Nat Prod. 2007 May;70(5):783-8. doi: 10.1021/np068074x. Epub 2007 May 4.
Six new cucurbitane glycosides, mogroside II B (2), 11-deoxymogroside III (4), 7-oxomogroside II E (5), 7-oxomogroside V (6), 11-oxomogroside II A1 (7), and 11-oxomogroside IV A (8), and two known but new naturally occurring cucurbitane glycosides, mogroside II A1 (1) and mogroside III A2 (3), were isolated from an ethanol extract of the fruits of Siraitia grosvenorii. Upon evaluation of compounds 1-8 for inhibitory effects against the Epstein-Barr virus early antigen (EBV-EA) activation induced by 12-O-tetradecanoylphorbol-13-acetate (TPA), all compounds exhibited inhibitory effects with IC50 values of 346-400 mol ratio/32 pmol TPA. In addition, compounds 1-8 showed weak inhibitory effects on activation of (+/-)-(E)-methyl-2-[(E)-hydroxyimino]-5-nitro-6-methoxy-3-hexemide (NOR 1), a nitric oxide (NO) donor.
从罗汉果果实的乙醇提取物中分离出6种新的葫芦烷糖苷，罗汉果苷II B（2）、11-脱氧罗汉果苷III（4）、7-氧代罗汉果苷II E（5）、7-氧代罗汉果苷V（6）、11-氧代罗汉果苷II A1（7）和11-氧代罗汉果苷IV A（8），以及2种已知但新发现的天然葫芦烷糖苷，罗汉果苷II A1（1）和罗汉果苷III A2（3）。在评估化合物1-8对由12-O-十四烷酰佛波醇-13-乙酸酯（TPA）诱导的爱泼斯坦-巴尔病毒早期抗原（EBV-EA）激活的抑制作用时，所有化合物均表现出抑制作用，IC50值为346-400摩尔比/32皮摩尔TPA。此外，化合物1-8对一氧化氮（NO）供体（+/-）-（E）-甲基-2-[(E)-羟基亚氨基]-5-硝基-6-甲氧基-3-己烯酰胺（NOR 1）的激活显示出微弱的抑制作用。
