Chu Chun-li, Deng Xiao-zhao, Zhang Yun, Dong Li-li, Wang Zhong-can, Yu Rong-bin, Wu Chao
School of Basic Medicine, Nanjing Medical University, Center of Disease Prevention and Control, Nanjing Command, Nanjing 210029, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2008 Apr;24(4):351-3, 364.
To investigate the diversity of proteins' pattern of the peripheral blood mononuclear cells (PBMCs) in patients with hepatitis C virus (HCV) infection, identify the differentially expressed proteins and analyze their roles in the mechanism of chronic hepatitis C.
The total proteins from PBMCs in HCV infected patients (28) and healthy persons (10) were separated by the immobilized pH gradient-based 2-DE. The differentially expressed proteins were screened by PDQuest analysis software, and then identified by peptide mass fingerprint (PMF) based on matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and database searching.
2-DE results showed that the mean number of protein spots in HCV infected patients and healthy persons was 625 and 614. Twelve differential proteins were tested by MALDI-TOF-MS, and 10 of them were identified. Some of the proteins participated in protein synthesis and degradation, signal transduction, metabolism, or cytoskeleton construction while some of them were proteins of virus.
2-DE pattern of PBMCs from HCV infected patients or healthy persons has been established and ten differentially expressed proteins has been characterized. Our study is helpful for clinical detection of HCV infection and further research into the mechanisms of chronic hepatitis C.
研究丙型肝炎病毒（HCV）感染患者外周血单个核细胞（PBMCs）蛋白质图谱的多样性，鉴定差异表达蛋白并分析其在慢性丙型肝炎发病机制中的作用。
采用基于固定化pH梯度的双向电泳（2-DE）分离HCV感染患者（28例）和健康人（10例）PBMCs中的总蛋白质。通过PDQuest分析软件筛选差异表达蛋白，然后基于基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）和数据库搜索，采用肽质量指纹图谱（PMF）进行鉴定。
2-DE结果显示，HCV感染患者和健康人蛋白质斑点的平均数分别为625个和614个。用MALDI-TOF-MS检测了12个差异蛋白，其中10个被鉴定。部分蛋白参与蛋白质合成与降解、信号转导、代谢或细胞骨架构建，部分为病毒相关蛋白。
建立了HCV感染患者和健康人PBMCs的2-DE图谱，并鉴定了10个差异表达蛋白。本研究有助于HCV感染的临床检测及慢性丙型肝炎发病机制的深入研究。
