Miwa Keishi, Misumi Kazuhiro, Shimada Kaoru, Miyoshi Noriaki, Inoue Takehiro, Kawabe Yoshihiro, Moriyama Kazuhiro, Ibara Satoshi, Ikenoue Tsuyomu, Sakamoto Hiroshi
New Frontiers Research Laboratories, Toray Industries Inc., Kanagawa, Japan.
J Perinat Med. 2007;35(3):232-5. doi: 10.1515/JPM.2007.045.
A novel blood purification material that we previously reported as a superantigen- and cytokine-adsorbing device (SCAD) was evaluated for its ability to adsorb unbound, unconjugated bilirubin (UUBil) in vitro and in vivo.
In albumin-containing buffer, UUBil was dissolved and circulated through the SCAD column. Also, bilirubin was infused into low-body weight newborn piglets and hemoperfused for 3 h over SCAD columns.
In albumin-containing buffer, concentration of bilirubin decreased from 34 to 0.6 mg/dL within 5 h and the SCAD fiber turned brown, indicating that bilirubin was adsorbed onto the surface of the adsorbent and was not degraded during the circulation. Using the hyperbilirubinemia swine, clearances of total bilirubin (TBil), direct bilirubin (DBil), and indirect bilirubin (IdBil) were significantly higher (P<0.01) in the SCAD group compared with the control group. The clearances of TBil, DBil, and IdBil at 3 h after the initiation of the bilirubin infusion were 0.47, 0.53, and 0.45 mL/min, respectively, at a blood flow rate of 2.5 mL/min, and this result indicates that almost 20% of bilirubins were adsorbed to the SCAD column in a single passage.
These results provide initial evidence that SCAD treatment is effective in the removal of UUBil and can be performed safely in newborn animals.
我们之前报道的一种新型血液净化材料，即超抗原和细胞因子吸附装置（SCAD），对其在体外和体内吸附未结合、非共轭胆红素（UUBil）的能力进行了评估。
在含白蛋白的缓冲液中溶解UUBil，并使其通过SCAD柱循环。此外，将胆红素注入低体重新生仔猪体内，并通过SCAD柱进行3小时的血液灌流。
在含白蛋白的缓冲液中，胆红素浓度在5小时内从34降至0.6mg/dL，SCAD纤维变为褐色，表明胆红素吸附在吸附剂表面，且在循环过程中未被降解。使用高胆红素血症猪，与对照组相比，SCAD组的总胆红素（TBil）、直接胆红素（DBil）和间接胆红素（IdBil）清除率显著更高（P<0.01）。在胆红素输注开始后3小时，当血流速度为2.5mL/min时，TBil、DBil和IdBil的清除率分别为0.47、0.53和0.45mL/min，这一结果表明单次通过时近20%的胆红素被吸附到SCAD柱上。
这些结果提供了初步证据，表明SCAD治疗在去除UUBil方面有效，并且可以在新生动物中安全进行。
