Wise Roger P, Moscou Matthew J, Bogdanove Adam J, Whitham Steven A
Corn Insects and Crop Genetics Research, USDA-ARS, Iowa State University, Ames, Iowa 50011-1020, USA.
Annu Rev Phytopathol. 2007;45:329-69. doi: 10.1146/annurev.phyto.45.011107.143944.
Using genomic technologies, it is now possible to address research hypotheses in the context of entire developmental or biochemical pathways, gene networks, and chromosomal location of relevant genes and their inferred evolutionary history. Through a range of platforms, researchers can survey an entire transcriptome under a variety of experimental and field conditions. Interpretation of such data has led to new insights and revealed previously undescribed phenomena. In the area of plant-pathogen interactions, transcript profiling has provided unparalleled perception into the mechanisms underlying gene-for-gene resistance and basal defense, host vs nonhost resistance, biotrophy vs necrotrophy, and pathogenicity of vascular vs nonvascular pathogens, among many others. In this way, genomic technologies have facilitated a system-wide approach to unifying themes and unique features in the interactions of hosts and pathogens.
利用基因组技术，现在有可能在整个发育或生化途径、基因网络以及相关基因的染色体定位及其推断的进化历史的背景下研究假设。通过一系列平台，研究人员可以在各种实验和田间条件下对整个转录组进行检测。对这些数据的解读带来了新的见解，并揭示了以前未被描述的现象。在植物与病原体相互作用的领域，转录谱分析为基因对基因抗性和基础防御、寄主与非寄主抗性、活体营养与死体营养以及维管束与非维管束病原体的致病性等潜在机制提供了无与伦比的认知，等等。通过这种方式，基因组技术促进了一种全系统的方法，以统一寄主与病原体相互作用中的主题和独特特征。
