Bioresource Institute for Healthy Utilization, Zunyi Medical University, Zunyi, Guizhou, China.
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China.
Bioengineered. 2021 Dec;12(1):1173-1188. doi: 10.1080/21655979.2021.1905325.
Transcriptome is used to determine the induction response of T2 plus line (abbreviated as line) infected with . The main objective of the study was to deal with the transcriptome database of line resistance to soft rot pathogens to provide a new perspective for identifying the resistance-related genes and understanding the molecular mechanism. Results indicated that water soaking and tissue collapse started at 20 h after line was infected by . A total of 1360 and 5768 differentially expressed genes (DEGs) were identified at 0 h and 20 h, respectively. After 20 h of infection, growth and development-related pathways were inhibited. Meanwhile, DEGs were promoted the colonization of pathogens in specific cell wall modification processes at the early infected stage. A shift to a defensive response was triggered at 0 h. A large number of DEGs were mainly up-controlled at 20 h and were substantially used in the pathogen recognition and the introduction of signal transformation cascades, secondary metabolites biosynthesis, pathogenic proteins activation, transcription aspects and numerous transporters. Furthermore, our data provided novel insights into the transcript reprogramming of line in response to infestation.
转录组用于确定感染 的 T2 加系（简称系）的诱导反应。本研究的主要目的是处理系抗软腐病病原体的转录组数据库，为鉴定抗性相关基因和理解分子机制提供新的视角。结果表明，系感染 后 20 小时开始出现浸水和组织崩溃。分别在 0 h 和 20 h 时鉴定到了 1360 个和 5768 个差异表达基因（DEGs）。感染 20 h 后，生长和发育相关途径受到抑制。同时，DEGs 促进了病原体在特定细胞壁修饰过程中的定植。在 0 h 时触发了向防御反应的转变。大量 DEGs 主要在 20 h 时被上调，并大量用于病原体识别和信号转化级联、次生代谢物生物合成、致病蛋白激活、转录方面和众多转运体的引入。此外，我们的数据为系响应侵染的转录重编程提供了新的见解。
