Crutcher K A, Neaderhauser J, Schmidt P, Weingartner J
Department of Neurosurgery, University of Cincinnati Medical Center, Ohio 45267.
Exp Neurol. 1991 Nov;114(2):228-36. doi: 10.1016/0014-4886(91)90039-f.
An in vitro assay to test for regional differences in neurite growth-promoting and growth-inhibiting factors in tissue sections of CNS tissue has been adapted to the use of postmortem human brain tissue. Frozen sections of the temporal lobe from victims of Alzheimer's disease were used as substrates for sympathetic neurite outgrowth in tissue culture. Tissue sections from a non-Alzheimer's brain were used as a control. Both explanted chick sympathetic ganglia and dissociated chick sympathetic neurons were cultured for 3 to 5 days on tissue sections in the presence of exogenous nerve growth factor. The dichotomy between gray and white matter portions of the tissue sections in supporting neurite outgrowth that was previously reported for fresh frozen human brain tissue was also found to persist in postmortem tissue. In addition, the total neurite outgrowth from explanted sympathetic ganglia was found to be significantly less on postmortem sections when compared with previous results obtained from fresh frozen tissue samples of epileptic tissue. Dissociated neurons exhibited neurite outgrowth on Alzheimer's sections that showed preferential growth on blood vessel segments but no affinity for senile plaques. The results suggest that there is some decline in the neurite growth-promoting ability of cortical gray matter obtained from postmortem-derived brains when compared with fresh tissue and that senile plaques do not represent sites of neurite stimulation in this in vitro system.
一种用于检测中枢神经系统组织切片中神经突生长促进因子和生长抑制因子区域差异的体外试验已适用于尸检后的人脑组织。取自阿尔茨海默病患者颞叶的冰冻切片被用作组织培养中交感神经突生长的底物。取自非阿尔茨海默病大脑的组织切片用作对照。在外源性神经生长因子存在的情况下，将移植的鸡交感神经节和离散的鸡交感神经元在组织切片上培养3至5天。先前报道的新鲜冰冻人脑组织在支持神经突生长方面组织切片灰质和白质部分之间的二分法在尸检组织中也持续存在。此外，与先前从癫痫组织新鲜冰冻组织样本获得的结果相比，发现移植的交感神经节在尸检切片上的总神经突生长明显减少。离散的神经元在阿尔茨海默病切片上表现出神经突生长，在血管段上显示出优先生长，但对老年斑没有亲和力。结果表明，与新鲜组织相比，取自尸检大脑的皮质灰质促进神经突生长的能力有所下降，并且在该体外系统中，老年斑不代表神经突刺激位点。
