克隆并测序编码人核糖体蛋白S25的cDNA。

Cloning and sequencing a cDNA encoding human ribosomal protein S25.

作者信息

Li M L, Latoud C, Center M S

机构信息

Center for Basic Cancer Research, Division of Biology, Kansas State University, Manhattan 66506.

出版信息

Gene. 1991 Nov 15;107(2):329-33. doi: 10.1016/0378-1119(91)90335-9.

DOI:10.1016/0378-1119(91)90335-9

PMID:1748303

Abstract

A full-length cDNA clone has been isolated from a cDNA library prepared from mRNA of adriamycin-resistant human leukemia HL60 cells. The nucleotide sequence of this cDNA has been determined and the protein coded for by the gene identified. The cDNA encodes a polypeptide of 125 amino acids (aa) with a deduced Mr of 13750. The deduced aa sequence of this protein has 56% homology to yeast ribosomal protein S31. Western-blot analysis using antibodies directed against a synthetic peptide based on the deduced aa sequence identifies the gene product as the human ribosomal protein S25.

摘要

已从用阿霉素抗性人白血病HL60细胞的mRNA制备的cDNA文库中分离出一个全长cDNA克隆。已确定该cDNA的核苷酸序列，并鉴定了该基因编码的蛋白质。该cDNA编码一个由125个氨基酸（aa）组成的多肽，推导的分子量为13750。该蛋白质推导的氨基酸序列与酵母核糖体蛋白S31有56%的同源性。使用基于推导的氨基酸序列针对合成肽的抗体进行的蛋白质印迹分析将该基因产物鉴定为人核糖体蛋白S25。