人核糖体蛋白S3a：cDNA的克隆及蛋白质的一级结构
Human ribosomal protein S3a: cloning of the cDNA and primary structure of the protein.
作者信息
Metspalu A, Rebane A, Hoth S, Pooga M, Stahl J, Kruppa J
机构信息
Estonian Biocenter, Tartu.
出版信息
Gene. 1992 Oct 1;119(2):313-6. doi: 10.1016/0378-1119(92)90289-2.
PMID:1398113
Abstract
The amino acid (aa) sequence of human ribosomal protein S3a (hRPS3a) was deduced partially from the nucleotide sequence of the corresponding cDNA and confirmed by direct aa sequencing from the N terminus of the purified hRPS3a protein. The cDNA clone was isolated from a cDNA expression library in the pEX vector using antibodies. The hRPS3a protein has 263 aa and its calculated M(r) is 29 813.
摘要
人类核糖体蛋白S3a（hRPS3a）的氨基酸（aa）序列部分地从相应cDNA的核苷酸序列推导得出，并通过从纯化的hRPS3a蛋白的N末端进行直接氨基酸测序得到证实。使用抗体从pEX载体中的cDNA表达文库中分离出该cDNA克隆。hRPS3a蛋白有263个氨基酸，其计算的分子量（M(r)）为29813。
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