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采用等电聚焦和免疫印迹法对血迹和精液进行转铁蛋白亚型分析。

Transferrin subtyping of bloodstains and semen using isoelectric focusing and immunoblotting.

作者信息

Yamaba T, Sawazaki K, Yasuda T, Nadano D, Iida R, Kishi K

机构信息

Scientific Investigation Laboratory, Fukui Prefectural Police Headquarters, Japan.

出版信息

Forensic Sci Int. 1991 Sep;50(2):187-94. doi: 10.1016/0379-0738(91)90150-h.

DOI:10.1016/0379-0738(91)90150-h
PMID:1748355
Abstract

Transferrin (TF) subtyping was carried out on bloodstains that had been made on cotton sheeting and stored under a variety of conditions ranging from -20 degrees C to +37 degrees C. The time limit of detection was longer than 54 weeks after dry storage under each condition. Moreover the correlation between isoprotein types of the TF in blood and semen samples from the same individual was determined in 103 men. All three TF common types and two rare types in all semen samples correlated with the type found in the corresponding blood sample. A combination of isoelectric focusing separation and immuno-enzyme-linked detection may prove to be very useful for forensic TF subtyping.

摘要

对在棉质床单上制作并在从-20摄氏度到+37摄氏度的各种条件下储存的血迹进行了转铁蛋白(TF)亚型分析。在每种条件下干燥储存后,检测的时间限制超过54周。此外,还对103名男性的血液和精液样本中TF的同功蛋白类型之间的相关性进行了测定。所有精液样本中的三种TF常见类型和两种罕见类型均与相应血液样本中发现的类型相关。等电聚焦分离和免疫酶联检测相结合可能被证明对法医TF亚型分析非常有用。

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