美法仑与热疗对慢性海洛因成瘾者遗传毒性的增强作用。

Enhanced genotoxicity by melphalan and hyperthermia in chronic heroin addicts.

作者信息

Vassiliades N, Mourelatos D, Eliopoulos P, Kourakis A, Dozi-Vassiliades J

机构信息

Department of Medicinal Biology and Genetics, Faculty of Medicine, Aristotelian University, Thessaloniki, Greece.

出版信息

Forensic Sci Int. 1991 Sep;50(2):269-76. doi: 10.1016/0379-0738(91)90158-f.

DOI:10.1016/0379-0738(91)90158-f

PMID:1748362

Abstract

Spontaneous melphalan- (MEL-) and MEL-hyperthermia- (MEL-HYP-) induced sister chromatid exchange (SCE) frequencies have been studied in 12 chronic heroin addicts (HER AD) and in 12 age- and sex-matched healthy controls. The incidence of spontaneous SCEs in lymphocytes from the HER AD was significantly greater (P less than 0.001) than those from the control subjects. When exposed to MEL in vitro, cells from both groups showed an increase in SCE frequency with yields from HER AD higher than those from controls. When exposed to MEL and hyperthermia (41.5 degrees C for 3 h) in vitro, cells from both groups showed further increases in SCE frequency with yields from HER AD higher than those from controls. We observed that hyperthermia (HYP) in combination with MEL synergistically enhances SCEs and cell division delays in both groups, with the synergistic effects in cells from HER AD higher than those from controls.

摘要

我们对12名慢性海洛因成瘾者（HER AD）和12名年龄及性别匹配的健康对照者进行了研究，观察了自发美法仑（MEL）及美法仑-热疗（MEL-HYP）诱导的姐妹染色单体交换（SCE）频率。HER AD淋巴细胞的自发SCE发生率显著高于对照者（P<0.001）。体外暴露于MEL时，两组细胞的SCE频率均增加，HER AD细胞的SCE产率高于对照者。体外暴露于MEL和热疗（41.5℃，3小时）时，两组细胞的SCE频率进一步增加，HER AD细胞的SCE产率高于对照者。我们观察到，热疗（HYP）与MEL联合使用可协同增强两组细胞的SCE并延迟细胞分裂，HER AD细胞中的协同效应高于对照者。