创伤弧菌rtxA的鉴定与特性分析，该基因对体外细胞毒性和小鼠体内毒力至关重要。
Identification and characterization of the Vibrio vulnificus rtxA essential for cytotoxicity in vitro and virulence in mice.
作者信息
Lee Jeong Hyun, Kim Myung Won, Kim Byoung Sik, Kim Seung Min, Lee Byung Cheol, Kim Tae Sung, Choi Sang Ho
机构信息
Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University, Seoul 151-742, Republic of Korea.
出版信息
J Microbiol. 2007 Apr;45(2):146-52.
A mutant exhibiting decreased cytotoxic activity toward INT-407 intestinal epithelial cells and carrying a mutation in the rtx gene cluster that consists of rtxCA and rtxBDE operons was screened from a library of V. vulnificus mutants. The functions of the rtxA gene, assessed by constructing an isogenic mutant and evaluating its phenotypic changes, demonstrated that RtxA is essential for the virulence of V. vulnificus in mice as well as in tissue cultures.
从创伤弧菌突变体文库中筛选出一株对INT-407肠上皮细胞细胞毒性活性降低且rtx基因簇发生突变的突变体，该基因簇由rtxCA和rtxBDE操纵子组成。通过构建同基因突变体并评估其表型变化来评估rtxA基因的功能，结果表明RtxA对于创伤弧菌在小鼠以及组织培养中的毒力至关重要。
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