Department of Infectious Diseases and Microbial Genomics, Nitte University Centre for Science Education and Research, Nitte (Deemed to be University), Mangaluru, India.
Center for Bioinformatics and Biostatistics, Nitte (Deemed to be University), Mangaluru, India.
Vet Q. 2023 Dec;43(1):1-17. doi: 10.1080/01652176.2023.2240389.
an opportunistic human pathogen native to marine/estuarine environment, is one of the leading causes of death due to seafood consumption and exposure of wounds to seawater worldwide. The present study involves the whole genome sequence analysis of an environmental strain of (clinical genotype) isolated from seafood along the Mangaluru coast of India. The sequenced genome data was subjected to analysis of phylogeny, virulence genes, antimicrobial resistance determinants, and secretary proteins using suitable bioinformatics tools. The sequenced isolate had an overall genome length of 4.8 Mb and GC content of 46% with 4400 coding DNA sequences. The sequenced strain belongs to a new sequence type (Multilocus sequence typing) and was also found to branch with a phylogenetic lineage that groups the most infectious strains of . The seafood isolate had complete genes involved in conferring serum resistance yet showed limited serum resistance. The study identified several genes against the antibiotics that are commonly used in their treatment, highlighting the need for alternative treatments. Also, the secretory protein analysis revealed genes associated with major pathways like ABC transporters, two-component systems, quorum sensing, biofilm formation, cationic antimicrobial peptide (CAMP) resistance, and others that play a critical role in the pathogenesis of the . To the best of our knowledge, this is the first report of a detailed analysis of the genomic information of a isolated from the Indian subcontinent and provides evidence that raises public health concerns about the safety of seafood.
一种机会主义的人类病原体，原产于海洋/河口环境，是全球因食用海鲜和伤口暴露于海水而导致死亡的主要原因之一。本研究涉及从印度芒格洛尔海岸的海鲜中分离出的一株（临床基因型）环境株的全基因组序列分析。对测序基因组数据进行了系统发育、毒力基因、抗菌药物耐药决定因素和分泌蛋白的分析，使用了合适的生物信息学工具。测序分离株的全基因组长度为 4.8Mb，GC 含量为 46%，有 4400 个编码 DNA 序列。测序菌株属于一个新的序列类型（多位点序列分型），也与一组最具传染性的 菌株的进化枝分支。该海鲜分离株具有完整的赋予血清抗性的基因，但表现出有限的血清抗性。研究发现了一些对抗生素有抗性的基因，这些抗生素是治疗中常用的，这突出了需要替代治疗方法。此外，分泌蛋白分析显示了与主要途径相关的基因，如 ABC 转运蛋白、双组分系统、群体感应、生物膜形成、阳离子抗菌肽（CAMP）抗性等，这些基因在 的发病机制中起着关键作用。据我们所知，这是首次对从印度次大陆分离出的 进行基因组信息详细分析的报告，并提供了证据，引起了人们对海鲜安全性的公共卫生关注。
