Quantitative proteomic analysis of mammalian organisms using metabolically labeled tissues.

Metabolic labeling of mammalian organisms with stable isotopes can be used to provide tissue-specific internal standards for use in quantitative proteomic analyses. This method provides an alternative and complementary strategy to covalent modification approaches using isotope-coded mass tags. This chapter will focus on the generation of the isotope-labeled tissues, the analysis of the sample using Multidimensional Protein Identification Technology, and the computational analysis of the mass spectrometric data acquired.