Gevaert Kris, Impens Francis, Ghesquière Bart, Van Damme Petra, Lambrechts Anja, Vandekerckhove Joël
Department of Medical Protein Research, VIB, Ghent University, Ghent, Belgium.
Proteomics. 2008 Dec;8(23-24):4873-85. doi: 10.1002/pmic.200800421.
Labeling of proteins and peptides with stable heavy isotopes (deuterium, carbon-13, nitrogen-15, and oxygen-18) is widely used in quantitative proteomics. These are either incorporated metabolically in cells and small organisms, or postmetabolically in proteins and peptides by chemical or enzymatic reactions. Only upon measurement with mass spectrometers holding sufficient resolution, light, and heavy labeled peptide ions or reporter peptide fragment ions segregate and their intensity values are subsequently used for quantification. Targeted use of these labels or mass tags further leads to specific monitoring of diverse aspects of dynamic proteomes. In this review article, commonly used isotope labeling strategies are described, both for quantitative differential protein profiling and for targeted analysis of protein modifications.
用稳定重同位素（氘、碳-13、氮-15和氧-18）标记蛋白质和肽在定量蛋白质组学中被广泛应用。这些同位素要么通过代谢整合到细胞和小生物体中，要么通过化学或酶促反应在蛋白质和肽的代谢后阶段进行标记。只有在使用具有足够分辨率的质谱仪进行测量时，轻标记和重标记的肽离子或报告肽片段离子才会分离，随后它们的强度值用于定量分析。有针对性地使用这些标记或质量标签进一步实现了对动态蛋白质组不同方面的特异性监测。在这篇综述文章中，描述了常用于定量差异蛋白质谱分析和蛋白质修饰靶向分析的同位素标记策略。
