de Monbrison Frédérique, Mihoubi Ilhem, Picot Stéphane
Service de Parasitologie, Hôpital E. Herriot, Hospices Civils de Lyon, France.
Acta Trop. 2007 May;102(2):79-83. doi: 10.1016/j.actatropica.2007.04.001. Epub 2007 Apr 6.
We evaluated the use of real-time PCR for the identification of cutaneous Leishmania species. The assay, based on the melting curve analysis of fluorescent products, allows the discrimination of four culture adapted strains (L. infantum MHOM/TN/80/IPT1, L. major MHOM/SU/73/5-ASKH, L. donovani MHOM/IN/80/DD8 and L. tropica MHOM/SU/74/K27). One hundred and twenty-nine skin lesions, spotted on filter paper, were collected from patients consulting for suspicion of cutaneous leishmaniasis (CL) at the parasitology laboratory of Constantine Hospital (Algeria). Ninety-seven (75.2%) of the samples analyzed were positive. Sixty-one (5%) were related to L. major strain. These results indicate that PCR assay provides pleasant results with filter paper and represents a tool for the identification of old world CL.
我们评估了使用实时聚合酶链反应（PCR）来鉴定皮肤利什曼原虫种类。该检测方法基于荧光产物的熔解曲线分析，能够区分四种适应培养的菌株（婴儿利什曼原虫MHOM/TN/80/IPT1、硕大利什曼原虫MHOM/SU/73/5-ASKH、杜氏利什曼原虫MHOM/IN/80/DD8和热带利什曼原虫MHOM/SU/74/K27）。从阿尔及利亚君士坦丁医院寄生虫学实验室怀疑患有皮肤利什曼病（CL）前来咨询的患者身上收集了129个点样在滤纸上的皮肤病变样本。分析的样本中有97个（75.2%）呈阳性。其中61个（5%）与硕大利什曼原虫菌株有关。这些结果表明，PCR检测方法在使用滤纸时能提供良好的结果，并且是鉴定旧大陆CL的一种工具。
