Ogasawara Yuki, Namai Tomoyuki, Yoshino Fumihiko, Lee Masaichi-Chang-il, Ishii Kazuyuki
Department of Environmental Biology, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.
FEBS Lett. 2007 May 29;581(13):2473-7. doi: 10.1016/j.febslet.2007.04.062. Epub 2007 Apr 30.
In this work, we examined the antioxidant role of mucin, a typical sialic acid containing high-molecular weight glycoprotein. The function of mucin as a hydroxyl radical (.OH) scavenger was characterized using bovine submaxillary gland mucin (BSM). Non-treated BSM effectively protected DNA from the attack of .OH; however, desialylated BSM lost this potential. Moreover, we estimated the scavenging effects of BSM against .OH generated by UV irradiation of hydrogen peroxide using ESR analysis. Our results indicate that BSM has .OH scavenging ability the and sialic acid in mucin is an essential moiety to scavenge .OH.
在本研究中，我们检测了粘蛋白的抗氧化作用，粘蛋白是一种典型的含唾液酸的高分子量糖蛋白。使用牛下颌下腺粘蛋白（BSM）对粘蛋白作为羟基自由基（·OH）清除剂的功能进行了表征。未处理的BSM能有效保护DNA免受·OH的攻击；然而，去唾液酸化的BSM失去了这种能力。此外，我们使用电子自旋共振分析评估了BSM对过氧化氢紫外线照射产生的·OH的清除效果。我们的结果表明，BSM具有·OH清除能力，且粘蛋白中的唾液酸是清除·OH的关键部分。
