Kwiatek Michał, Majka Maciej, Wiśniewska Halina, Apolinarska Barbara, Belter Jolanta
Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznań, Poland,
J Appl Genet. 2015 May;56(2):163-8. doi: 10.1007/s13353-014-0264-3. Epub 2014 Dec 14.
This paper shows the results of effective uses of a molecular cytogenetics toolbox and molecular marker to transfer leaf rust resistance genes from Aegilops tauschii × Secale cereale (DDRR, 2n = 4x = 28) amphiploid forms to triticale cv. Bogo (AABBRR, 2n = 6x = 42). The molecular markers of resistance genes and in situ hybridization analysis of mitotic metaphase of root meristems confirmed the stable inheritance of chromosome 3D segments carrying Lr32 from the BC2F2 to the BC2F5 generation of (Ae. tauschii × S. cereale) × triticale hybrids. The chromosome pairing analysis during metaphase I of meiosis of BC2F4 and BC2F5 hybrids showed increasing regular bivalent formation of 3D chromosome pairs and decreasing number of univalents in subsequent generations. The results indicate that using amphiploid forms as a bridge between wild and cultivated forms can be a successful technology to transfer the D-genome chromatin carrying leaf rust resistance genes into triticale.
本文展示了有效利用分子细胞遗传学工具箱和分子标记将叶锈病抗性基因从节节麦×黑麦（DDRR，2n = 4x = 28）双二倍体形式转移到小黑麦品种Bogo（AABBRR，2n = 6x = 42）的结果。抗性基因的分子标记以及根分生组织有丝分裂中期的原位杂交分析证实，携带Lr32的3D染色体片段从（节节麦×黑麦）×小黑麦杂种的BC2F2代到BC2F5代实现了稳定遗传。BC2F4和BC2F5杂种减数分裂中期I的染色体配对分析表明，在后代中3D染色体对的规则二价体形成增加，单价体数量减少。结果表明，利用双二倍体形式作为野生和栽培形式之间的桥梁，可能是将携带叶锈病抗性基因的D基因组染色质转移到小黑麦中的一项成功技术。
