为期两天的放射状臂水迷宫学习和记忆任务；转基因小鼠中淀粉样蛋白相关记忆缺陷的有效解决。

Two-day radial-arm water maze learning and memory task; robust resolution of amyloid-related memory deficits in transgenic mice.

作者信息

Alamed Jennifer, Wilcock Donna M, Diamond David M, Gordon Marcia N, Morgan Dave

机构信息

Alzheimer's Research Laboratory, Department of Molecular Pharmacology and Physiology, School of Basic Biomedical Sciences, College of Medicine, Tampa, Florida 33612, USA.

出版信息

Nat Protoc. 2006;1(4):1671-9. doi: 10.1038/nprot.2006.275.

DOI:10.1038/nprot.2006.275

PMID:17487150

Abstract

The radial arm water maze (RAWM) contains six swim paths (arms) extending out of an open central area, with an escape platform located at the end of one arm (the goal arm). The goal arm location remains constant for a given mouse. On day 1, mice are trained for 15 trials (spaced over 3 h), with trials alternating between visible and hidden platform. On day 2, mice are trained for 15 trials with the hidden platform. Entry into an incorrect arm is scored as an error. The RAWM has the spatial complexity and performance measurement simplicity of the dry radial arm maze combined with the rapid learning and strong motivation observed in the Morris water maze without requiring foot shock or food deprivation as motivating factors. With two sessions each day, 16 mice can be tested over 2 days.

摘要

放射状臂水迷宫（RAWM）有六条从开放的中央区域延伸出来的游泳路径（臂），其中一个臂的末端设有逃生平台（目标臂）。对于给定的小鼠，目标臂的位置保持不变。在第1天，小鼠接受15次训练（间隔3小时），训练在可见平台和隐藏平台之间交替进行。在第2天，小鼠用隐藏平台进行15次训练。进入错误的臂被计为一次错误。放射状臂水迷宫具有干式放射状臂迷宫的空间复杂性和性能测量简单性，同时具备莫里斯水迷宫中观察到的快速学习能力和强烈动机，且无需以足部电击或食物剥夺作为激励因素。每天进行两个实验环节，16只小鼠可在两天内完成测试。