Bromley-Brits Kelley, Deng Yu, Song Weihong
Department of Psychiatry, Brain Research Center, University of British Columbia.
J Vis Exp. 2011 Jul 20(53):2920. doi: 10.3791/2920.
The Morris Water Maze (MWM) was first established by neuroscientist Richard G. Morris in 1981 in order to test hippocampal-dependent learning, including acquisition of spatial memory and long-term spatial memory. The MWM is a relatively simple procedure typically consisting of six day trials, the main advantage being the differentiation between the spatial (hidden-platform) and non-spatial (visible platform) conditions. In addition, the MWM testing environment reduces odor trail interference. This has led the task to be used extensively in the study of the neurobiology and neuropharmacology of spatial learning and memory. The MWM plays an important role in the validation of rodent models for neurocognitive disorders such as Alzheimer's Disease. In this protocol we discussed the typical procedure of MWM for testing learning and memory and data analysis commonly used in Alzheimer's disease transgenic model mice.
莫里斯水迷宫（MWM）由神经科学家理查德·G·莫里斯于1981年首次建立，用于测试海马体依赖的学习，包括空间记忆的获取和长期空间记忆。MWM是一个相对简单的程序，通常包括为期六天的试验，其主要优点是能够区分空间（隐藏平台）和非空间（可见平台）条件。此外，MWM测试环境减少了气味痕迹干扰。这使得该任务在空间学习和记忆的神经生物学和神经药理学研究中得到广泛应用。MWM在验证诸如阿尔茨海默病等神经认知障碍的啮齿动物模型方面发挥着重要作用。在本方案中，我们讨论了用于测试学习和记忆的MWM典型程序以及阿尔茨海默病转基因模型小鼠常用的数据分析方法。
