用构建的大肠杆菌-长双歧杆菌穿梭载体pRM2转化长双歧杆菌。
Transformation of Bifidobacterium longum with pRM2, a constructed Escherichia coli-B. longum shuttle vector.
作者信息
Missich R, Sgorbati B, LeBlanc D J
机构信息
Laboratory of Microbial Genetics, University of Bologna, Italy.
出版信息
Plasmid. 1994 Sep;32(2):208-11. doi: 10.1006/plas.1994.1056.
PMID:7846144
Abstract
An Escherichia coli-Bifidobacterium longum shuttle vector, designated pRM2, was constructed by cloning a B. longum plasmid and an enterococcal spectinomycin resistance gene into a commercial E. coli vector. The plasmid was successfully introduced into B. longum cells by electroporation and into E. coli cells by both electroporation and chemical transformation.
摘要
一种名为pRM2的大肠杆菌-长双歧杆菌穿梭载体，是通过将长双歧杆菌质粒和肠球菌壮观霉素抗性基因克隆到一种商业化的大肠杆菌载体中构建而成的。该质粒通过电穿孔成功导入长双歧杆菌细胞，并通过电穿孔和化学转化导入大肠杆菌细胞。
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