Kumar Ravindra, El-Sagheer Afaf, Tumpane John, Lincoln Per, Wilhelmsson L Marcus, Brown Tom
School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK.
J Am Chem Soc. 2007 May 30;129(21):6859-64. doi: 10.1021/ja070273v. Epub 2007 May 9.
The copper-catalyzed azide-alkyne cycloaddition reaction has been used for the template-mediated chemical ligation of two oligonucleotide strands, one with a 5'-alkyne and the other with a 3'-azide, to produce a DNA strand with an unnatural backbone at the ligation point. A template-free click-ligation reaction has been used for the intramolecular circularization of a single stranded oligonucleotide which was used as a template for the synthesis of a covalently closed DNA catenane.
铜催化的叠氮化物-炔烃环加成反应已被用于两条寡核苷酸链的模板介导化学连接，一条链带有5'-炔基，另一条链带有3'-叠氮基，以在连接点产生具有非天然主链的DNA链。一种无模板的点击连接反应已被用于单链寡核苷酸的分子内环化，该单链寡核苷酸用作合成共价闭合DNA连环体的模板。
