R&D Center, Rznomics Inc., Seongnam 13486, Korea.
Department of Bioconvergence Engineering, Research Institute of Advanced Omics, Dankook University, Yongin 16890, Korea.
Int J Mol Sci. 2022 Oct 31;23(21):13247. doi: 10.3390/ijms232113247.
mRNA is gaining success as a new therapeutic agent and vaccine. However, mRNA has limitations in stability. To overcome the shortcomings of mRNA, circular RNA is emerging as a new modality. In this review, several current methods of manufacturing circular RNA in vitro are introduced and their advantages and disadvantages are reviewed. Furthermore, this study discusses which fields and directions of research and development are needed for the increase in the efficacy and productivity of circular RNA as a therapeutic agent and vaccine formulation.
mRNA 作为一种新的治疗药物和疫苗正在取得成功。然而，mRNA 在稳定性方面存在局限性。为了克服 mRNA 的缺点，环状 RNA 作为一种新的模式正在出现。在这篇综述中，介绍了几种体外制备环状 RNA 的常用方法，并对其优缺点进行了评价。此外，本研究还讨论了在提高环状 RNA 作为治疗药物和疫苗制剂的疗效和生产力方面需要增加哪些研究和开发领域和方向。
