Gonçalves Roberto Martins, Rodrigues David Henrique, Camargos da Costa Alinne Maria, Teixeira Mauro Martins, Ribeiro Campos Wesley, Oréfice Fernando, Teixeira Antônio Lúcio
Department of Ophthalmology, Faculty of Medicine, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Acta Ophthalmol Scand. 2007 Dec;85(8):871-6. doi: 10.1111/j.1600-0420.2007.00943.x. Epub 2007 May 4.
Chemokines have been implicated in the control of leucocyte infiltration in uveitis and in modulating angiogenesis in several ocular conditions. Toxoplasmic retinochoroiditis is a common cause of posterior uveitis. This study aimed to evaluate the serum concentrations of CC and CXC chemokines in patients with acute toxoplasmic retinochoroiditis.
The levels of five chemokines (CCL2, CCL11, CXCL9, CXCL8 and CXCL10) were evaluated in the serum of patients with active toxoplasmic retinochoroiditis (n = 55) and control subjects (n = 40). In a subset of patients (n = 18), a second measure of serum levels of chemokines was performed after the completion of oral treatment with pyrimethamine (25 mg/day), sulphadiazine (1 g, four times per day), folinic acid (7.5 mg/day) and prednisone (initial dose: 1 mg/kg/day) for approximately 30 days.
Patients with toxoplasmic retinochoroiditis, notably those presenting with vasculitis, had increased serum levels of CXCL8 (mean +/- standard error of the mean [SEM] 35.1 +/- 6.5 pg/ml) compared with control subjects (mean +/- SEM 16.0 +/- 2.3 pg/ml; p = 0.01). There were no differences between patients and controls in serum levels of the other chemokines measured. The size of ocular lesions correlated significantly with serum levels of CXCL8 and CXCL9. After treatment, there was a significant reduction in serum levels of CXCL8. Severity of vitreous opacities did not correlate with serum levels of these chemokines.
These data suggest a role for CXCL8 in the inflammatory process of acute toxoplasmic retinochoroiditis. Furthermore, CXCL8 may be a useful marker for patient follow-up.
趋化因子与葡萄膜炎中白细胞浸润的控制以及多种眼部疾病中血管生成的调节有关。弓形虫性视网膜脉络膜炎是后葡萄膜炎的常见病因。本研究旨在评估急性弓形虫性视网膜脉络膜炎患者血清中CC和CXC趋化因子的浓度。
对活动性弓形虫性视网膜脉络膜炎患者（n = 55）和对照受试者（n = 40）的血清中五种趋化因子（CCL2、CCL11、CXCL9、CXCL8和CXCL10）的水平进行评估。在一部分患者（n = 18）中，在用乙胺嘧啶（25 mg/天）、磺胺嘧啶（1 g，每日4次）、亚叶酸（7.5 mg/天）和泼尼松（初始剂量：1 mg/kg/天）进行约30天的口服治疗结束后，再次测量血清趋化因子水平。
与对照受试者（平均±平均标准误[SEM] 16.0±2.3 pg/ml；p = 0.01）相比，弓形虫性视网膜脉络膜炎患者，尤其是那些出现血管炎的患者，血清CXCL8水平升高（平均±SEM 35.1±6.5 pg/ml）。所检测的其他趋化因子的血清水平在患者和对照之间没有差异。眼部病变的大小与CXCL8和CXCL9的血清水平显著相关。治疗后，CXCL8的血清水平显著降低。玻璃体混浊的严重程度与这些趋化因子的血清水平无关。
这些数据表明CXCL8在急性弓形虫性视网膜脉络膜炎的炎症过程中起作用。此外，CXCL8可能是患者随访的有用标志物。
