Hintao J, Teanpaisan R, Chongsuvivatwong V, Ratarasan C, Dahlen G
Epidemiology Unit, Faculty of Medicine, Prince of Songkla University, Thailand.
Oral Microbiol Immunol. 2007 Jun;22(3):175-81. doi: 10.1111/j.1399-302X.2007.00341.x.
The relationships between suspected bacteria in saliva, yeasts in oral rinse, and supragingival and subgingival plaque versus root surface and coronal caries in adults with type 2 diabetes mellitus and a non-diabetic group were explored.
One-hundred and five patients with type 2 diabetes and 103 non-diabetic subjects were recruited; their periodontal status, plaque index and magnitude of root surface and coronal caries were assessed. Saliva and an oral rinse were cultured for mutans streptococci, lactobacilli and yeasts. Toothbrush samples of supragingival plaque and curette samples of subgingival plaque were assessed for 17 bacterial species using the checkerboard DNA-DNA hybridization method.
Type 2 diabetes patients had significantly more severe periodontitis, a higher plaque index and a higher prevalence and magnitude of root surface caries than non-diabetic subjects. Significantly more diabetic subjects had higher levels of Treponema denticola, Prevotella nigrescens, Streptococcus sanguinis, Streptococcus oralis and Streptococcus intermedius in their supragingival plaque than non-diabetic subjects. No significant difference was found for the organisms in saliva, oral rinse and subgingival plaque between the two groups. After adjustment for diabetic status, root surface caries was associated with an increased count of mutans streptococci, lactobacilli and yeasts in saliva and of Streptococcus mutans in supragingival plaque samples. Coronal caries was only associated with lactobacilli and yeasts in saliva.
The number of cariogenic organisms in saliva and oral rinse estimated by culture demonstrated a stronger association with both root surface and coronal caries compared to those 17 species assessed with the checkerboard method in supragingival and subgingival plaque.
探讨了2型糖尿病成年患者和非糖尿病组中，唾液中的可疑细菌、漱口水样本中的酵母菌与龈上和龈下菌斑相对于根面龋和冠龋之间的关系。
招募了105例2型糖尿病患者和103例非糖尿病受试者；评估了他们的牙周状况、菌斑指数以及根面龋和冠龋的严重程度。对唾液和漱口水样本进行变形链球菌、乳酸菌和酵母菌培养。使用棋盘式DNA-DNA杂交法评估龈上菌斑的牙刷样本和龈下菌斑的刮治样本中的17种细菌。
与非糖尿病受试者相比，2型糖尿病患者的牙周炎更严重，菌斑指数更高，根面龋的患病率和严重程度也更高。龈上菌斑中，糖尿病受试者的具核梭杆菌、变黑普氏菌、血链球菌、口腔链球菌和中间链球菌水平显著高于非糖尿病受试者。两组在唾液、漱口水和龈下菌斑中的微生物方面未发现显著差异。在调整糖尿病状态后，根面龋与唾液中变形链球菌、乳酸菌和酵母菌数量增加以及龈上菌斑样本中变形链球菌数量增加有关。冠龋仅与唾液中的乳酸菌和酵母菌有关。
通过培养估计的唾液和漱口水样本中致龋微生物数量，与通过棋盘式方法评估的龈上和龈下菌斑中的17种细菌相比，与根面龋和冠龋的相关性更强。
