Huskić Jasminko, Culo Filip, Dautović Sajma, Mulabegović Nedzad
Institute of Physiology and Biochemistry, Faculty of Medicine, University of Sarajevo, Cekalusa 90, 71000 Sarajevo, Bosnia and Herzegovina.
Bosn J Basic Med Sci. 2007 Feb;7(1):33-6. doi: 10.17305/bjbms.2007.3086.
Angiotensin converting enzyme (ACE) and nitric oxide (NO) have been suggested to be involved in the regulation of fluid homeostasis. In the present investigation, ACE activity and NO levels were determined in serum of 20 patients (10 men and 10 women) with dehydration caused by gastroenterocolitis and 20 healthy individuals (10 men and 10 women). Serum and tissue ACE activity was determined by spectrophotometric method using hippuryl-l-histidyl-l-leucine (Hip-His-Leu) as a substrate. NO synthesis was determined by measuring the products of NO, nitrite and nitrate. The concentration of nitrites was determined by classic colorimetric method using Griess reagent. Nitrate concentration was determined indirectly by their reduction with elementary zinc into nitrite. Results have shown that serum ACE activity in patients with dehydration (36,46+/-2,74 U/L) is statistically higher then in healthy individuals (28,71+/-1,77 U/L, p<0,05). The average level of nitrites/nitrates in serum of patients with dehydration (30,57+/-1,05 microM; mean +/- SEM) is also statistically higher then in healthy individuals (12,44+/-0,60 microM, p<0,0001). There was no correlation between ACE activity and NO production. The results indicate that ACE and NO may participate in the regulation of the alteration in blood flow and in the regulation of the water balance in patients with dehydration.
血管紧张素转换酶（ACE）和一氧化氮（NO）被认为参与了体液平衡的调节。在本研究中，测定了20例因胃肠结肠炎导致脱水的患者（10名男性和10名女性）以及20名健康个体（10名男性和10名女性）血清中的ACE活性和NO水平。血清和组织中的ACE活性采用分光光度法，以马尿酰-L-组氨酰-L-亮氨酸（Hip-His-Leu）为底物进行测定。通过测量NO的产物亚硝酸盐和硝酸盐来测定NO的合成。亚硝酸盐浓度采用经典比色法，使用格里斯试剂进行测定。硝酸盐浓度通过用单质锌将其还原为亚硝酸盐来间接测定。结果表明，脱水患者血清中的ACE活性（36.46±2.74 U/L）在统计学上高于健康个体（28.71±1.77 U/L，p<0.05）。脱水患者血清中亚硝酸盐/硝酸盐的平均水平（30.57±1.05 μM；均值±标准误）在统计学上也高于健康个体（12.44±0.60 μM，p<0.0001）。ACE活性与NO生成之间无相关性。结果表明，ACE和NO可能参与了脱水患者血流变化的调节以及水平衡的调节。
