Babić Nermina, Huskić Jasminko, Nakas-Ićindić Emina
Institute of Physiology and Biochemistry, Faculty of Medicine, University of Sarajevo, Cekalusa 90, 71000 Sarajevo, Bosnia and Herzegovina.
Bosn J Basic Med Sci. 2007 Feb;7(1):79-83. doi: 10.17305/bjbms.2007.3098.
Serum and tissue (kidney's) angiotensin-converting enzyme (ACE) activity has been examined in Wistar rats (10 males and 10 females), seven days after unilateral nephrectomy. Renal hypertrophy was determined by measurement of kidney absolute mass. Serum and tissue ACE activity was determined by spectrophotometric method using hippuryl-l-histidyl-l-leucine (Hip-His-Leu) as a substrate. The ACE serum activity was expressed in units that correspond to 1 nmol of hippuric acid released by enzymatic hydrolysis of Hip-His-Leu substrate per minute/ml serum. The ACE tissue activity was expressed in units that correspond to 1 nmol of hippuric acid released by enzymatic hydrolysis of Hip-His-Leu substrate per minute/mg protein or mg kidney's tissue. The ACE serum activity significantly increased (p<0,05) seven days after unilateral nephrectomy. The ACE tissue activity, expressed in units that corresponds to 1 nmol of hippuric acid released by hydrolysis of Hip-His-Leu substrate per minute/mg protein, was higher seven days after unilateral nephrectomy then in kidney control, but the difference was not significant compared to the values determined in kidney control. The ACE tissue activity, expressed in units that correspond to 1 nmol of hippuric acid released by hydrolysis of Hip-His-Leu substrate per minute/mg tissue, was increased seven days after unilateral nephrectomy, which is statistically significant compared to the activity of the same enzyme in kidney control (p<0,01). The results indicate that ACE, probably has an important role in development of adaptive compensatory mechanisms after unilateral nephrectomy.
在单侧肾切除术后七天，对Wistar大鼠（10只雄性和10只雌性）的血清和组织（肾脏）血管紧张素转换酶（ACE）活性进行了检测。通过测量肾脏绝对质量来确定肾肥大。采用分光光度法，以马尿酰-L-组氨酰-L-亮氨酸（Hip-His-Leu）为底物测定血清和组织中的ACE活性。血清ACE活性以每分钟每毫升血清中通过酶促水解Hip-His-Leu底物释放1 nmol马尿酸的单位来表示。组织ACE活性以每分钟每毫克蛋白质或毫克肾脏组织中通过酶促水解Hip-His-Leu底物释放1 nmol马尿酸的单位来表示。单侧肾切除术后七天，血清ACE活性显著增加（p<0.05）。以每分钟每毫克蛋白质中通过水解Hip-His-Leu底物释放1 nmol马尿酸的单位表示的组织ACE活性，在单侧肾切除术后七天高于肾脏对照组，但与肾脏对照组测定的值相比差异不显著。以每分钟每毫克组织中通过水解Hip-His-Leu底物释放1 nmol马尿酸的单位表示的组织ACE活性，在单侧肾切除术后七天增加，与肾脏对照组中同一酶的活性相比具有统计学意义（p<0.01）。结果表明，ACE可能在单侧肾切除术后适应性代偿机制的发展中起重要作用。
